Regulatory B Cells : Methods and Protocols / edited by Francesca Mion, Silvia Tonon.

Format:

Book

Contributor:

Mion, Francesca, Editor.

Tonon, Silvia, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2270

Methods in Molecular Biology, 1940-6029 ; 2270

Language:

English

Subjects (All):

Immunology.

Local Subjects:

Immunology.

Physical Description:

1 online resource (XIV, 471 pages) : 90 illustrations, 66 illustrations in color.

Edition:

2nd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This second edition provides new and updated chapters useful for the study of Regulatory B cells. Organized in four sections, chapters detail basic methods for the isolation and immunophenotypical analysis of these cells, experimental approaches for the ex vivo generation/expansion of IL-10 producing B cells, molecular biology techniques for the analysis of IL-10 expression and production, and animal models mimicking pathologic settings. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Regulatory B Cells: Methods and Protocols aims to be useful to the scientific community and serve to clarify some unsolved aspects of Regulatory B Cells research.

Contents:

Purification and characterization of murine MZ and T2-MZP cells

Purification and immune-phenotyping of B-1 cells from body cavities of mice

Purification and immunophenotypic characterization of murine plasma cells

Purification of murine and human IL-10 producing B cells from different anatomical compartments

Purification and immunophenotypic characterization of human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ B cells

Detection of IL-10 in murine B cells: in vitro and in vivo techniques

Detection and quantification of transforming growth factor-β1 produced by murine B cells: pros and cons of different techniques

IL-35 detection in B cells at the mRNA and protein level

Characterization and activation of Fas Ligand producing mouse B cells and their killer exosomes

Characterization and activity of TIM-1 and IL-10-reporter expressing regulatory B cells

New method for the expansion of highly purified human regulatory granzyme B-expressing B cells

Characterization of the cell surface phenotype and regulatory activity of B cell IgD low (BDL) regulatory B cells

Use of Toll-like receptor (TLR) ligation to characterize human regulatory B cells subsets

Use of co-cultures for the study of cellular interactions influencing B cell regulatory functions

Use of inhibitory compounds to dissect the molecular pathways involved in regulatory B cell differentiation

Methods to study the transcriptome of regulatory B cells

Purification of murine IL-10+ B cells for analyses of biological functions and transcriptomics

il-10 gene locus DNA methylation in regulatory B cells

B cell commitment to IL-10 production: the Vert-X Il10egfp mouse

Regulatory B cells in experimental mouse models of arthritis

Regulatory and IgE+ B cells in allergic asthma

Regulatory B cells in type 1 diabetes

Analysis of regulatory B cells in experimental autoimmune uveitis

Purification and immunophenotypic characterization of human CD24hiCD38hi and CD24hiCD27+ regulatory B cells in transplantation.

Other Format:

Printed edition:

ISBN:

978-1-0716-1237-8

9781071612378

Access Restriction:

Restricted for use by site license.