Germline Development in the Zebrafish : Methods and Protocols / edited by Roland Dosch.

Format:

Book

Contributor:

Dosch, Roland, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2218

Methods in Molecular Biology, 1940-6029 ; 2218

Language:

English

Subjects (All):

Cytology.

Cell Biology.

Local Subjects:

Cell Biology.

Physical Description:

1 online resource (XIV, 383 pages) : 87 illustrations, 76 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume details a wide range of methods, ranging from beginner through advanced, used to further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish

The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins

Computer-Assisted Sperm Analysis to Test Environmental Toxicants

Cryopreservation and Transplantation of Spermatogonial Stem Cells

Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos

Chemical Genetics: Manipulating the Germline with Small Molecules

In vitro Induction of Teleost PGCs

Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization

Cryopreservation of Pooled Sperm Samples

Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery

In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads

Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries

Detection of the Polar Body after Fertilization

Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles

Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ Cells

Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos

Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy

Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo

Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining

Histological Analysis of Gonads in Zebrafish

Manipulating and Visualizing the Germline with Transgenic Lines

Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs

In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish

In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation

The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life

Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach

Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos

-Massively Parallel Analysis of Regulatory RNA Sequences

Exploring Translational Control of Maternal mRNAs in Zebrafish. .

Other Format:

Printed edition:

ISBN:

978-1-0716-0970-5

9781071609705

Access Restriction:

Restricted for use by site license.