The Complement System : Innovative Diagnostic and Research Protocols / edited by Lubka T. Roumenina.

Format:

Book

Contributor:

Roumenina, Lubka T., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2227

Methods in Molecular Biology, 1940-6029 ; 2227

Language:

English

Subjects (All):

Diseases.

Local Subjects:

Diseases.

Physical Description:

1 online resource (XII, 267 pages) : 55 illustrations, 41 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume describes a range of methods to be used in complement laboratories use and how to interpret the data. Chapters detail methods for depletion of IgG and IgM, quantification of complement proteins, C3dg quantification, complement C3 deposition on endothelial cells, anti-C1q auto-antibodies, and methods for assessment of interactions of proteins with heme. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and clearly written, The Complement System: Innovative Diagnostic and Research Protocols aims to ensure successful results in the further study of this vital field.

Contents:

The Benefits of Complement Measurements for the Clinical Practice

Method for Depletion of IgG and IgM from Human Serum as Naive Complement Source

Quantification of Complement Proteins with Special Reference to C1q: Multiplex vs ELISA vs Rocket Immunoelectrophoresis vs Nephelometry

C3dg Quantification by PEG-precipitation and ELISA or TRIFMA

Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope Based Enzyme-Linked Immunosorbent Assay

Sheep Erythrocytes Preparation for Hemolytic Tests Exploring Complement Functional Activities

Hemolytic Tests Exploring Factor H Functional Activities

Functional Hemolytic Test for Complement Alternative Pathway Convertase Activity

Complement C3 Deposition on Endothelial Cells Revealed by Flow Cytometry

Anti-C1q Auto-Antibodies: Standard Quantification and Innovative ELISA

Anti-C1-Inhibitor Auto-Antibodies Detection by ELISA

Anti-ficolin-2 and anti-ficolin-3 Autoantibodies Detection by ELISA

Detection of anti-C3b Autoantibodies by ELISA

Detection of Complement Factor B Autoantibodies by ELISA

Detection of C3 Nephritic Factor by Hemolytic Assay

Detection of Genetic Rearrangements inthe Regulators of Complement Activation RCA Cluster by High Throughput Sequencing and MLPA

Complement Detection in Mouse Kidneys by Immunofluorescence

Complement Detection in Human Tumors by Immunohistochemistry and Immunofluorescence

Analysis Of The Ligand Recognition Specificities Of Human Ficolins Using Surface Plasmon Resonance

Methods for Assessment of Interactions of Proteins with Heme - Application for Complement Proteins and Immunoglobulins

Evaluation of Binding Kinetics and Thermodynamics of Antibody-antigen Interactions and Interactions Involving Complement Proteins

Purification of Human Complement Component C4 and Sample Preparation for Structural Biology Applications. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1016-9

9781071610169

Access Restriction:

Restricted for use by site license.