Plant Transposable Elements : Methods and Protocols / edited by Jungnam Cho.

Format:

Book

Contributor:

Cho, Jungnam, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2250

Methods in Molecular Biology, 1940-6029 ; 2250

Language:

English

Subjects (All):

Plant genetics.

Plant Genetics.

Local Subjects:

Plant Genetics.

Physical Description:

1 online resource (XV, 272 pages) : 61 illustrations, 46 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.

Contents:

A Predictive Approach to Infer the Activity and Natural Variation of Retrotransposon Families in Plants

In silico Identification of Stress Associated Transposable Elements in Arabidopsis thaliana using Public Transcriptome Data

A Practical Guide on Computational Tools and Databases for Transposable Elements in Plants

A Tutorial of EDTA: Extensive de-novo TE Annotator

Mining of Miniature Transposable Elements in Brassica species at BrassicaTED

Genome-wide Identification of Miniature Inverted-Repeat Transposable Elements by Targeted High-Throughput Sequencing

Identification of Extrachromosomal Circular Forms of Active Transposable Elements Using Mobilome-Seq

Identification of Active Transposable Elements in Plants: The mobilome-seq Approach

High-throughput Profiling of Extrachromosomal Linear DNAs of Long Terminal Repeat Retrotransposons by ALE-seq

Bioinformatics Analysis Guides to LTR Retrotransposon-Derived Extrachromosomal Linear DNAs Identified by ALE-seq

Determination of TE insertion Positions using Transposon Display

Detection of Transposition Events from Next-Generation Sequencing Data

Rapid Detection of Transposon-Induced Genome Rearrangements

Detection of Transposable Element Insertions in Arabidopsis Using Sequence Capture

Efficient Detection of Transposable Element Insertion Polymorphisms Between Genomes Using Short-Read Sequencing Data

Quantitative Measurement of Transposon Copy Number using the Droplet Digital PCRDetecting Signatures of TE Polymorphisms in Short-read Sequencing Data

How to Activate Heat-responsible Retrotransposon ONSEN in Brassicaceae Species

Measurement of Genetic Mobility Using a Transposon-Based Marker System In Sorghum

Sequence-specific Amplified Polymorphism (SSAP) and Sequence Characterized Amplified Region (SCAR) Markers in Zea mays

Analysis of Plant DNA Methylation Profiles using R

Arabidopsis thaliana Mature Endosperm Dissection and Isolation of Seed Tissue Genomic DNA

Transposon-Based Tagging in Silico using FastPCR Software

Bamboo Transposon Research: Current Status and Perspectives. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1134-0

9781071611340

Access Restriction:

Restricted for use by site license.