Mass Spectrometry-Based Lipidomics : Methods and Protocols / edited by Fong-Fu Hsu.

Format:

Book

Contributor:

Hsu, Fong Fu, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2306

Methods in Molecular Biology, 1940-6029 ; 2306

Language:

English

Subjects (All):

Lipids.

Mass spectrometry.

Lipidology.

Mass Spectrometry.

Local Subjects:

Lipidology.

Mass Spectrometry.

Physical Description:

1 online resource (XI, 314 pages) : 91 illustrations, 62 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This detailed volume covers conventional MS-based "shotgun lipidomics" by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

Contents:

Mass Spectrometry-Based Lipidomics: An Overview

High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry

Global Lipidomics Profiling by a High Resolution LC-MS Platform

Comprehensive Structural Characterization of Lipids by Coupling Paternò-Büchi Reaction and Tandem Mass Spectrometry

Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis

Quantitative Analysis of Polyphosphoinositide, Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun Lipidomics after Methylation

A Mass Spectrometry-Based Shotgun Lipidomics Using Charge-Switch Derivatization for Analysis of the Complex Long-Chain Fatty Acids

One-Pot Extractive Transesterification of Fatty Acids Followed by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS

Ceramide Analysis by Multiple Linked-Scan Mass Spectrometry Using a Tandem Quadrupole Instrument

Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High Resolution Mass Spectrometry Platform

Mass Spectrometric Analysis of Meibomian Gland Lipids

Quantification of Plasma Oxylipins Using Solid Phase Extraction and Reversed-Phase Liquid Chromatography-Triple Quadrupole Mass Spectrometry

Targeted Lipidomics of Drosophila melanogaster During Development

Shotgun Lipidomic Analysis of Leishmania Cells

Characterization of the Uncommon Lipid Families in Corynebacterium glutamicum by Mass Spectrometry

Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi

Analytical Methodologies for Lipidomics in Hemp Plant

Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry

Imaging of Polar and Nonpolar Lipids Using Desorption Electrospray Ionization/Post-Photoionization Mass Spectrometry

Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Lipids in the Ischemic Rat Brain Section: A Practical Approach.

Other Format:

Printed edition:

ISBN:

978-1-0716-1410-5

9781071614105

Access Restriction:

Restricted for use by site license.