Ras Activity and Signaling : Methods and Protocols / edited by Ignacio Rubio, Ian Prior.

Format:

Book

Contributor:

Rubio, Ignacio, Editor.

Prior, Ian, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2262

Methods in Molecular Biology, 1940-6029 ; 2262

Language:

English

Subjects (All):

Proteins.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Physical Description:

1 online resource (XII, 439 pages) : 86 illustrations, 57 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.

Contents:

Ras Variant Biology and Contributions to Human Disease

Regulation of the Small GTPase Ras and its Relevance to Human Disease

Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry

Absolute Quantitation of GTPase Protein Abundance

Validation of Isoform- and Mutation-specific RAS Antibodies

Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b

Active GTPase Pulldown Protocol

Methods to Monitor Ras Activation State

NMR Detection Methods for Profiling RAS Nucleotide Cycling

Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations

Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations

Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets

FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage

Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry

Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA)

Using BioID to Characterize the RAS Interactome

Probing RAS function with Monobodies

Detection of Endogenous RASSF1A Interacting Proteins

Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition

A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background

RASless MEFs as a Tool to Study RAS-dependent and -independent Functions

Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies

Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling

Studying Metabolic Abnormalities in the Costello Syndrome HRAS G12V Mouse Model: Isolation of Mouse Embryonic Fibroblasts and Their In Vitro Adipocyte Differentiation

Dissecting Oncogenic RAS Signalling in Melanoma Development in Genetically Engineered Zebrafish Models

Ras, Ral, and Rap1 in C. elegans. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1190-6

9781071611906

Access Restriction:

Restricted for use by site license.