The Myc Gene : Methods and Protocols / edited by Laura Soucek, Jonathan Whitfield.

Format:

Book

Contributor:

Soucek, Laura, Editor.

Whitfield, Jonathan, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2318

Methods in Molecular Biology, 1940-6029 ; 2318

Language:

English

Subjects (All):

Genetics.

Genetics and Genomics.

Local Subjects:

Genetics and Genomics.

Physical Description:

1 online resource (XIV, 349 pages) : 53 illustrations, 44 illustrations in color.

Edition:

2nd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This second edition provides new and updated chapters detailing recent advances in MYC research and current techniques. Chapters guide readers through protocols on how to express and purify MYC protein, X-ray crystallography, NMR, techniques to study how MYC is modified, apoptosis, senescence, proliferation, metabolic changes, translation, tumorigenesis,reprogramming, and clinical application of MYC studies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Myc Gene: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Contents:

An "-omycs" Toolbox to Work with MYC

Methods of Expression, Purification and Preparation of the c-Myc b-HLH-LZ for its Biophysical Characterization

Biophysical and Structural Methods to Study the bHLHzip Region of Human c-MYC

Identifying and Validating MYC:protein Interactors in Pursuit of Novel anti-MYC Therapies

Detection of Post-Translational Modifications on MYC

MYC Analysis in Cancer and Evolution

Genome-wide analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing

Analysis of Myc Chromatin Binding by Calibrated ChIP-seq Approach

A High-Throughput Chromatin Immunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape

Methods for Determining Myc-induced Apoptosis

Measuring MYC-mediated Metabolism in Tumorigenesis

Methods to study Myc-regulated Cellular Senescence - An Update

Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer

A Versatile in vivo System to Study Myc in Cell Reprogramming

Using Flow Cytometry to Study Myc's Role in Shaping the Tumor Immune Microenvironment

Generation of a Tetracycline Regulated Mouse Model of MYC-Induced T-Cell Acute Lymphoblastic Leukemia

Chromogenic in Situ Hybridization (CISH) as a Method for Detection of C-Myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue: An Update

MYC Copy Number Detection in Clinical Samples using a Digital DNA-Hybridization and Detection Method

Cell-Based Methods for the Identification of Myc-Inhibitory Small Molecules.

Other Format:

Printed edition:

ISBN:

978-1-0716-1476-1

9781071614761

Access Restriction:

Restricted for use by site license.