Myofibroblasts : Methods and Protocols / edited by Boris Hinz, David Lagares.

Format:

Book

Contributor:

Hinz, Boris, Editor.

Lagares, David, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2299

Methods in Molecular Biology, 1940-6029 ; 2299

Language:

English

Subjects (All):

Cytology.

Regenerative medicine.

Pharmacology.

Cell Biology.

Regenerative Medicine and Tissue Engineering.

Local Subjects:

Cell Biology.

Regenerative Medicine and Tissue Engineering.

Pharmacology.

Physical Description:

1 online resource (XV, 460 pages) : 86 illustrations, 83 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on fundamental methods to study myofibroblast biology and covers topics such as methods for detecting myofibroblasts and senescent myofibroblasts in cell culture and histology, single cell RNA sequencing to identify myofibroblast subsets in fibrotic tissues, and functional assays to assess TGF-β activation, myofibroblast apoptosis, or matrix deposition and crosslinking. Section II discusses methods to investigate the mechanobiology of myofibroblasts in vitro, including the fabrication of functional hydrogels with tunable stiffness, the use of atomic force microscopy to characterize matrix and cellular stiffness, as well as molecular assays to assess fibroblast mechanotransduction pathways and durotaxis. Section III describes multiple animal models to investigate myofibroblast functions across organs in vivo as well as human organoid systems, precision tissue slices and decellularized 3D tissue scaffolds to assess myofibroblast functions in relevant human ex vivo models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and extensive, Myofibroblasts: Methods and Protocols is an essential collection for researchers delving into the processes and effects of these important cells.

Contents:

50 Years of Myofibroblasts: How the Myofibroblast Concept Evolved

Myofibroblast Functions in Tissue Repair and Fibrosis: An Introduction

Myofibroblast Markers and Microscopy Detection Methods in Cell Culture and Histology

Fibroblast and Myofibroblast Subtypes: Single Cell Sequencing

Myofibroblast Adhesome Analysis by Mass Spectrometry

Myofibroblast TGF-β Activation Measurement In Vitro

Contraction Measurements Using Three-Dimensional Fibrillar Collagen Gel Lattices

Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro

Methods for Studying Myofibroblast Apoptotic Pathways

Determination of Senescent Myofibroblasts in Precision-Cut Lung Slices

The Scar-in-a-Jar: In Vitro Fibrosis Model for Anti-Fibrotic Drug Testing

Why Stress Matters: An Introduction

Soft Substrate Culture to Mechanically Control Cardiac Myofibroblast Activation

Quantitative Analysis of Myofibroblast Contraction by Traction Force Microscopy

Nucleocytoplasmic Shuttling of the Mechanosensitive Transcription Factors MRTF and YAP/TAZ

Atomic Force Microscopy for Live-Cell and Hydrogel Measurement

Method for Investigating Fibroblast Durotaxis

Decellularized Extracellular Matrix (ECM) as a Model to Study Fibrotic ECM Mechanobiology

Fibrosis on a Chip for Screening of Anti-Fibrosis Drugs

Animal and Human Models of Tissue Repair and Fibrosis: An Introduction

Mouse Models of Lung Fibrosis

Mouse Models of Kidney Fibrosis

Mouse Models of Liver Fibrosis

Mouse Models of Muscle Fibrosis

Mouse Models of Skin Fibrosis

Mouse Models of Intestinal Fibrosis

A Rodent Model of Hypertrophic Scarring: Splinting of Rat Wounds

Three-Dimensional Model of Hypertrophic Scar Using a Tissue-Engineering Approach

Methods for the Study of Renal Fibrosis in Human Pluripotent Stem Cell-Derived Kidney Organoids

Decellularized Human Lung Scaffolds as Complex Three-Dimensional Tissue Culture Models to Study Functional Behavior of Fibroblasts.

Other Format:

Printed edition:

ISBN:

978-1-0716-1382-5

9781071613825

Access Restriction:

Restricted for use by site license.