1 option
Confocal Microscopy : Methods and Protocols / edited by Joseph Brzostowski, Haewon Sohn.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2304
- Methods in Molecular Biology, 1940-6029 ; 2304
- Language:
- English
- Subjects (All):
- Biophysics.
- Spectrum analysis.
- Cytology.
- Bioanalysis and Bioimaging.
- Spectroscopy.
- Cell Biology.
- Local Subjects:
- Bioanalysis and Bioimaging.
- Spectroscopy.
- Cell Biology.
- Physical Description:
- 1 online resource (XI, 363 pages) : 88 illustrations, 73 illustrations in color.
- Edition:
- 1st ed. 2021.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2021.
- System Details:
- text file PDF
- Summary:
- This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
- Contents:
- Advances in Confocal Microscopy and Selected Applications
- Choosing Fluorescent Probes and Labeling Systems
- General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy
- Microfabricated devices for confocal microscopy on biological samples
- Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging
- High-resolution multicolor imaging of mitochondria in lymphocytes
- Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy
- Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy
- Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight
- Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum
- Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis
- High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains
- Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy
- Studying Neuronal Biology Using Spinning Disc Confocal Microscopy
- Method for Acute Intravital Imaging of the Large Intestine in Live Mice
- Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism
- Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry
- FLIM Imaging for Metabolic Studies in Live Cells
- A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM).
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-1402-0
- 9781071614020
- Access Restriction:
- Restricted for use by site license.
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.