Plant Lipids : Methods and Protocols / edited by Dorothea Bartels, Peter Dörmann.

Format:

Book

Contributor:

Bartels, Dorothea, Editor.

Dormann, Peter, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2295

Methods in Molecular Biology, 1940-6029 ; 2295

Language:

English

Subjects (All):

Botany.

Lipids.

Plant Science.

Lipidology.

Local Subjects:

Plant Science.

Lipidology.

Physical Description:

1 online resource (XIV, 472 pages) : 82 illustrations, 52 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally. .

Contents:

Lipid Isolation from Plants

Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and Yield

Thin-Layer Chromatography

Lipid Analysis by Gas Chromatography and Gas Chromatography Mass Spectrometry

14C-Tracing of Lipid Metabolism

Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes

Direct Infusion Mass Spectrometry for Complex Lipid Analysis

Fatty Acid Composition by Total-Acyl-Lipid

Collision Induced Dissociation

Time-of-Flight (TAL-CID-TOF) Mass Spectrometry

Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS

Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism

Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MS

Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae

Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS

Structural Analysis of Glycosylglycerolipids using NMR Spectroscopy

Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and Seeds

Isolation of Lipid Droplets for Protein and Lipid Analysis

Isolation of Plastoglobules for Lipid Analyses

Isolation of Mitochondria for Lipid Analysis

Collection and Analysis of Phloem Lipids

Analyses of Inositol Phosphates and Phosphoinositides by Strong Anion Exchange (SAX)-HPLC

Analysis of Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption Chromatography and Subsequent Quantification via Thin-Layer and Gas Chromatography

Studying Lipid-Protein Interactions Using Protein-Lipid-Overlay and Protein-Liposome-Association Assays

Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC)

In Situ Localization of Plant Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI)

Plant Lipid Databases

Lipid Pathway Databases with a Focus on Algae.

Other Format:

Printed edition:

ISBN:

978-1-0716-1362-7

9781071613627

Access Restriction:

Restricted for use by site license.