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Mitochondrial Medicine : Volume 2: Assessing Mitochondria / edited by Volkmar Weissig, Marvin Edeas.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2276
- Methods in Molecular Biology, 1940-6029 ; 2276
- Language:
- English
- Subjects (All):
- Medicine-Research.
- Biology-Research.
- Biomedical Research.
- Local Subjects:
- Biomedical Research.
- Physical Description:
- 1 online resource (XVI, 459 pages) : 112 illustrations, 75 illustrations in color.
- Edition:
- 2nd ed. 2021.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Springer, 2021.
- System Details:
- text file PDF
- Summary:
- This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional' mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.
- Contents:
- Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow
- Preparation of 'Functional' Mitochondria - A Challenging Business
- Isolation and Quality Control of Functional Mitochondria
- Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient
- Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues
- Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems
- Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes
- Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging
- Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays
- Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues
- Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria
- Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence
- Assessment of Mitochondrial Ca2+ Uptake
- Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices
- Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis
- Analysis of Mitochondrial Dysfunction during Cell Death
- Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes
- Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane
- Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway
- 3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction
- Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology
- High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria
- Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism
- Fluorescence-based Assay for Measuring OMA1 Activity
- Studying Mitochondrial Network Formation by in vivo and in vitro Reconstitution Assay
- Extraction of Functional Mitochondria Based on the Membrane Stiffness
- A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing
- A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry
- In vivo Visualization And Quantification of Mitochondrial Morphology in C. elegans
- Assessing Impact of Platinum Complexes on Mitochondrial Functions
- In-silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models
- Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a New Molecular Biosensor.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-1266-8
- 9781071612668
- Access Restriction:
- Restricted for use by site license.
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