Non-Alcoholic Steatohepatitis : Methods and Protocols / edited by Devanand Sarkar.

Format:

Book

Contributor:

Sarakāra, Debānanda, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2455

Methods in Molecular Biology, 1940-6029 ; 2455

Language:

English

Subjects (All):

Medical genetics.

Stem cells.

Clinical Genetics.

Stem Cell Biology.

Local Subjects:

Clinical Genetics.

Stem Cell Biology.

Physical Description:

1 online resource (XIII, 361 pages) : 79 illustrations, 68 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; protocols for isolating hepatocytes and Kupffer cells, bone marrow derived macrophages, and adipocytes; techniques to develop human pluripotent stem cells-derived liver organoids; single-cell and RNA-sequencing; and a description of how to extract exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols is a valuable resource for new and experiences investigators studying NASH, and serves as an essential reference on NASH for basic and clinical researchers and students. .

Contents:

Histopathological Diagnosis of Nonalcoholic Steatohepatitis (NASH)

Generation of a Diet-Induced Mouse Model of Nonalcoholic Fatty Liver Disease

Hydrodynamic Injection for Developing NASH Model

Measurement of Hepatic Lipids

Measurement of Fatty Acid Oxidation in Mammalian Cells

Measurement of In Vivo VLDL and Chylomicron Secretion

Isolation and Culture of Mouse Hepatocytes and Kupffer Cells (KC)

Mouse Bone Marrow Cell Isolation and Macrophage Differentiation

Purification and Isolation of Hepatic Stellate Cells

Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into White or Beige Adipocytes

Culture of Mouse Liver Ductal Organoids

Development of a Scalable Three-Dimensional Culture of Human Induced Pluripotent Stem Cells-Derived Liver Organoids

Chromatin Immunoprecipitation Assay in Primary Mouse Hepatocytes and Mouse Liver

Analysis of Liver Responses to Non-Alcoholic Steatohepatitis by mRNA-Sequencing

Single-Cell RNA-Sequencing in NASH

Computational Pipeline for Next-Generation Sequencing (NGS) Studies in Genetics of NASH

Metabolic Phenotyping in Mice with NASH using Indirect Calorimetry

Analysis of Insulin Resistance in Nonalcoholic Steatohepatitis

Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH)

Ex Vivo Dual-Hit Method for Inflammasome Activation in Liver

In Vivo Analysis of Necrosis and Ferroptosis in Nonalcoholic Steatohepatitis (NASH)

Analysis of the Sphingolipidome in NAFLD

Bile Acid Profiling in Mouse Biofluids and Tissues

Preparation and Characterization of a Liver Targeted, Poly(amidoamine) Based, Gene Delivery System

Extraction of Exosomes and Exosomal miRNA from Mesenchymal Stem Cells

Generation of Adenovirus for In Vitro and In Vivo Studies of Hepatocytes.

Other Format:

Printed edition:

ISBN:

978-1-0716-2128-8

9781071621288

Access Restriction:

Restricted for use by site license.