Fluorescent Microscopy / edited by Bryan Heit.

Format:

Book

Contributor:

Heit, Bryan, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2440

Methods in Molecular Biology, 1940-6029 ; 2440

Language:

English

Subjects (All):

Materials-Microscopy.

Microscopy.

Local Subjects:

Microscopy.

Physical Description:

1 online resource (XII, 370 pages) : 97 illustrations, 70 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field. .

Contents:

Fluorescence Microscopy: A Field Guide for Biologists

Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy

Optimizing Long-Term Live Cell Imaging

Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy

Bimolecular Fluorescence Complementation to Visualize Protein:Protein Interactions in Cells

Monitoring Cellular Responses to Infection with Fluorescent Biosensors

Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF)

Measurement of Minute Cellular Forces by Traction Force Microscopy

Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues

Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin

Quantifiable Intravital Light Sheet Microscopy

Hydrophobic and Hydrogel-based Methods for Passive Tissue Clearing

Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos

Super Resolution Radial Fluctuations (SRRF) Microscopy

A Practical Guide for STED Imaging

Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons

Measuring the Lateral Diffusion of Plasma Membrane Receptors using Raster Image Correlation Spectroscopy

Nanometer-Scale Molecular Mapping by Super-Resolution Fluorescence Microscopy

Visualizing and Quantifying Data from Timelapse Imaging Experiments

Automated Segmentation and Microscopy Image Analysis with Machine Learning.

Other Format:

Printed edition:

ISBN:

978-1-0716-2051-9

9781071620519

Access Restriction:

Restricted for use by site license.