Stem Cell Assays : Methods and Protocols / edited by Nagarajan Kannan, Philip Beer.

Format:

Book

Contributor:

Kannan, Nagarajan, Editor.

Beer, Philip, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2429

Methods in Molecular Biology, 1940-6029 ; 2429

Language:

English

Subjects (All):

Stem cells.

Stem Cell Biology.

Local Subjects:

Stem Cell Biology.

Physical Description:

1 online resource (XVII, 569 pages) : 123 illustrations, 92 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume explores the fields of stem cell biology, regenerative medicine, and cancer biology. The chapters in this book cover topics such as embryonic stem cells (ES) and induced pluripotent cells (iPS); ES/iPSC derived tissue stem cells; tissue resident stem cells; assays for primitive malignant cells; and CRISPR/Cas9 engineering and transgenic model development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cell Assays: Methods and Protocols is a valuable laboratory manual that allows researchers and scientists to learn about new techniques and approaches in this developing field.

Contents:

Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cells

Artificial Activation of Murine Oocytes using Strontium to Derive Haploid and Diploid Parthenotes

Generation of Human iPSC from Small Volume Peripheral Blood Samples

Distinguishing between Endodermal and Pluripotent Stem Cell Lines during Somatic Cell Reprogramming

Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

Detection of Soluble and Insoluble Protein Species in Patient-Derived iPSCs

Extracellular Flux Analysis of Mitochondrial Function in Pluripotent Stem Cells

Assessment of Endothelial-to-Hematopoietic Transition of Individual Hemogenic Endothelium and Bulk Populations in Defined Conditions

Homogenous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells

Differentiation of Human Induced Pluripotent Stem Cells into Cortical Neurons to Advance Precision Medicine

Differentiation of iPS-Cells into Peripheral Sensory Neurons

Culture of Human iPSC-Derived Motoneurons in Compartmentalized Microfluidic Devices and Quantitative Assays for Studying Axonal Phenotypes

iPS Cell-Differentiation into Brain Microvascular Endothelial Cells

Chromatin Immunoprecipitation in Human Pluripotent Stem Cell-Derived 3D Organoids to Analyze DNA-Protein Interactions

Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells

Generation of Salivary Gland Organoids from Mouse Embryonic Stem Cells

In Vitro Generation of Heart Field Specific Cardiomyocytes

Isolation and Characterization of Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells

Identification and Validation of CRISPR/Cas9 Off-Target Activity in Hematopoietic Stem and Progenitor Cells

Genome Engineering of Hematopoietic Stem Cells using CRISPR/Cas9 System

Generation of Rat Neural Stem Cells to Produce Different Astrocyte Phenotypes

In Situ Quantification and Isolation of Müller Glial Cells by Fluorescence Activated Cell Sorting from the Regenerating Larval Zebrafish Retina

Quantification and Cloncal Culture of Neural Stem Cells from the Hippocampus of Adult Mouse

Reprogramming Mouse Oviduct Epithelial Cells using In Vivo Electroporation and CRISPR/Cas9 Mediated Genetic Manipulation

Generation of Human Liver Chimeric Mice and Harvesting of Human Hepatocytes from Mouse Livers

Application of 3D Culture Assays to Study Breast Morphogenesis, Epithelial Plasticity, and Cellular Interactions in an Epithelial Progenitor Cell Line

A Unified Protocol to Streamline Molecular and Cellular Analysis for Three-Dimensional Cell Cultures

Mesenchymal Stem Cell Seeding on 3D Scaffolds

Assaying Candidate Human Skin Keratinocyte Stem Cells by Determining their Long-Term Serial Proliferative Output in Culture

Protocol for the Detection of Organoid-Initiating Cell Activity in Patience-Derived Single Fallopian Tube Epithelial Cells

Quantification of Mucle Stem Cell Differentiation using Live-Cell Imaging and Eccentricity Measures

The Enrichment of Breast Cancer Stem Cells from MCF7 Breast Cancer Cell Line using Spheroid Culture Technique

Assessment of Breast Cancer Stem Cell Activity using a Spheroid Formation Assay

Enrichment of Cancer Stem Cells in a Tumor Sphere Assay

In Vitro Quantification of Cancer Stem Cells using a Mammosphere Formation Assay

Designing Genetically Engineered Mouse Models (GEMMSs) using CRISPS Mediated Genome Editing

Assays for the Spectrum of Circulating Tumor Cells

Limiting Dilution Tumor Initiation Assay: An In Vitro Approach for the Study of Cancer Stem Cells

Orthotopic Xenografts of Colorectal Cancer Stem Cells.

Other Format:

Printed edition:

ISBN:

978-1-0716-1979-7

9781071619797

Access Restriction:

Restricted for use by site license.