Mammalian Cell Engineering : Methods and Protocols / edited by Ryosuke Kojima.

Format:

Book

Contributor:

Kojima, Ryosuke, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2312

Methods in Molecular Biology, 1940-6029 ; 2312

Language:

English

Subjects (All):

Biotechnology.

Local Subjects:

Biotechnology.

Physical Description:

1 online resource (XI, 330 pages) : 83 illustrations, 67 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols is a valuable resource that allows scientists to successfully carry out their research, thus ultimately contributing to the future advancement of this field.

Contents:

Generation of CAR-T Cells by Lentiviral Transduction

Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cells

Engineering Mammalian Cells to Control Glucose Homeostasis

Using Engineered Mammalian Cells for an Epitope-Directed Antibody Affinity Maturation System

Purification of Specific Cell Populations Differentiated from Stem Cells using MicroRNA-Responsive Synthetic Messenger RNAs

Green Light-Controlled Gene Switch for Mammalian and Plant Cells

Sonogenetic Modulation of Cellular Activities in Mammalian Cells

Constructing Smartphone-Controlled Optogenetic Switches in Mammalian Cells

Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice

Construction of Caffeine-Inducible Gene Switches in Mammalian Cells

Multiplexed Genome Engineering with Cas12a

Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells

Optical Control of Genome Editing by Photoactivatable Cas9

Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT

Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules

AgDD System: A Chemical Controllable Protein Aggregates in Cells

Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme

Feeder-Free Human Induced Pluripotent Stem Cell Culture using a DNA Aptamer-Based Mimic of Basic Fibroblast Growth Factor

Protocol for de novo Gene Targeting via In Utero Electroporation

Magnetically Guided Single Cell Virus Stamping. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1441-9

9781071614419

Access Restriction:

Restricted for use by site license.