Mycobacteria Protocols / edited by Tanya Parish, Anuradha Kumar.

Format:

Book

Contributor:

Parish, Tanya, Editor.

Kumar, Anu, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2314

Methods in Molecular Biology, 1940-6029 ; 2314

Language:

English

Subjects (All):

Microbiology.

Pathology.

Local Subjects:

Microbiology.

Pathology.

Physical Description:

1 online resource (XV, 736 pages) : 104 illustrations, 80 illustrations in color.

Edition:

4th ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This fully updated edition explores the latest techniques to study the challenging, and at times dangerous, genus of bacteria known as mycobacteria with basic methods that are still required for mycobacteriology along with the newer or improved methods that have been developed. The volume features chapters on the basics of DNA isolation, protein isolation, and lipid isolation, as well as more sophisticated techniques for isolation of ribosomes, and continues with sections involving analyzing subcellular fractions, culture methods, sequencing technology, in vitro models, molecular methods, as well as drug discovery applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mycobacteria Protocols, Fourth Edition serves as an ideal guide for those starting out with their mycobacteria research and also for those who have worked with it for decades.

Contents:

Culturing Mycobacteria

DNA Isolation from Mycobacteria

Extraction and Separation of Mycobacterial Proteins

Lipid and Lipoarabinomannan Isolation and Characterization

Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria

Macrophage Infection Models for Mycobacterium tuberculosis

Novel Single-Cell and High Throughput Microscopy Techniques to Monitor Dictyostelium discoideum-Mycobacterium marinum Infection Dynamics

Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy

Measuring Efflux and Permeability in Mycobacteria

Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria

Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells

Electroporation of Mycobacteria

Gene Switching and Essentiality Testing

Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis

Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action

CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria

Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology

Metabolic Labeling of Live Mycobacteria with Trehalose-Based Probes

Identification and Characterization of Mycobacterial Species Using Whole Genome Sequences

Whole Genome Sequencing of Mycobacterium tuberculosis Directly from Sputum Samples

Experimental and Computational Workflow for RNA Sequencing in Mycobacterium tuberculosis: From Total RNA to Differentially Expressed Genes

RNA Sequencing for Transcript 5'-End Mapping in Mycobacteria

Methods for Proteomic Analyses of Mycobacteria

Targeted Lipidomics of Mycobacterial Lipids and Glycolipids

Metabolomics of Mycobacterium tuberculosis

Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium

A Multi-Stress Model for High Throughput Screening Against Non-Replicating Mycobacterium tuberculosis

Antimicrobial Susceptibility Testing for Mycobacterium sps in High Throughput Format

High-Content Analysis Monitoring Intracellular Trafficking and Replication of Mycobacterium tuberculosis Inside Host Cells

Efficient Measurement of Drug Interactions with DiaMOND (Diagonal Measurement of N-Way Drug Interactions)

Drug Sensitivity Testing of Mycobacterium tuberculosis Growing in a Hollow Fiber Bioreactor.

Other Format:

Printed edition:

ISBN:

978-1-0716-1460-0

9781071614600

Access Restriction:

Restricted for use by site license.