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Plant Phosphoproteomics : Methods and Protocols / edited by Xu Na Wu.

SpringerProtocols (1984- current) Available online

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Format:
Book
Contributor:
Wu, Xu Na, Editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1940-6029 ; 2358
Methods in Molecular Biology, 1940-6029 ; 2358
Language:
English
Subjects (All):
Botany.
Cytology.
Plant Science.
Cell Biology.
Local Subjects:
Plant Science.
Cell Biology.
Physical Description:
1 online resource (XI, 241 pages) : 29 illustrations, 26 illustrations in color.
Edition:
1st ed. 2021.
Contained In:
Springer Nature eBook
Place of Publication:
New York, NY : Springer US : Imprint: Humana, 2021.
System Details:
text file PDF
Summary:
This detailed protocol book provides phosphoproteomics techniques currently developed for use in plants, as well as offering a variety of methodologies to analyze different types of plant phosphoproteomic data. Suitable protocols for every stage of the experiment, from protein isolation and phosphopeptides enrichment to large-scale data analysis, have been considered and explored. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Phosphoproteomics: Methods and Protocols serves as an ideal guide for researchers examining phosphoproteomics in the field of plant sciences.
Contents:
Phosphorylation Site Motifs in Plant Protein Kinases and Their Substrates
Protein Phosphorylation Response to Abiotic Stress in Plants
Protein Phosphorylation in Plant Cell Signaling
Phosphoproteomics Profiling of Receptor Kinase Mutants
Phosphoproteomics Analysis of Soybean Roots Under Salinity by Using the iTRAQ Labeling Approach
Universal Sample Preparation Workflow for Plant Phosphoproteomic Profiling
Mapping Plant Phosphoproteome with Improved Tandem MOAC and Label-Free Quantification
SILIA-Based 4C Quantitative PTM Proteomics
Phosphoproteomics Analysis of Plant Root Tissue
Plant Phosphopeptides Enrichment by Immobilized Metal Ion Affinity Chromatography
Two-Dimensional Gel Electrophoresis and Pro-Q Diamond Phosphoprotein Stain-Based Plant Phosphoproteomics
2-D DIGE Combined with Pro-Q Diamond Staining for the Identification of Protein Phosphorylation for Chlamydomonas reinhardtii: A Successful Approach
Plant Phosphopeptides Identification and Label-Free Quantification by MaxQuant and Proteome Discovery Software
Phosphat 4.0: An Updated Arabidopsis Database for Searching Phosphorylation Sites and Kinase-Target Interactions
Computational Phosphorylation Network Reconstruction: An Update on Methods and Resources
The Application of an R Language-Based Platform cRacker for Phosphoproteomics Data Analysis
Kinase Activity Assay Using Unspecific Substrate or Specific Synthetic Peptides.
Other Format:
Printed edition:
ISBN:
978-1-0716-1625-3
9781071616253
Access Restriction:
Restricted for use by site license.

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