Fe-S Proteins : Methods and Protocols / edited by Patricia C. Dos Santos.

Format:

Book

Contributor:

Dos Santos, Patricia C., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2353

Methods in Molecular Biology, 1940-6029 ; 2353

Language:

English

Subjects (All):

Proteins.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Physical Description:

1 online resource (XI, 351 pages) : 87 illustrations, 64 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume explores current technologies used to investigate the formation, insertion, and function of metalloclusters associated with proteins. Chapters describe relevant topics about Fe-S cluster metabolism are explored through genetic, biochemical, spectroscopic methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fe-S Proteins: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Contents:

Molecular biology and genetic tools to investigate functional redundancy among Fe-S cluster carriers in E. coli

Study Fe-S cluster proteins in Methanococcus maripaludis, a model archaeal organism

Genetic approaches to uncover gene products involved in iron-sulfur protein maturation: High throughput genomic screening using transposon-sequencing

Methods for heterologous overproduction of Fe-S proteins

Enzymatic and Chemical in vitro Reconstitution of Iron-Sulfur Cluster Proteins

Expression, isolation, and characterization of vanadium nitrogenase from Azotobacter vinelandii

Ni-NTA affinity chromatography to characterize protein-protein interactions during Fe-S cluster biogenesis

tRNA modifications as a readout of S and Fe-S metabolism

Methods to unravel the roles of ATPases in Fe-S cluster biosynthesis

Methods to investigate the kinetic profile of cysteine desulfurases

A fast and ratiometric method for quantification of cysteine-bound persulfides based on alkylation and gel-shift assays

Circular dichroism to probe the synthesis, transfer, and stability of Fe-S clusters

Native mass spectrometry of iron-sulfur proteins

Characterization of Paramagnetic Iron-Sulfur Clusters using Electron Paramagnetic Resonance Spectroscopy

Characterization of Fe-S clusters in proteins by Mӧssbauer Spectroscopy

Biochemical approaches to probe the role of the auxiliary iron-sulfur cluster of lipoyl synthase from Mycobacterium tuberculosis

Methods to Screen for Radical SAM Enzyme Crystallization Conditions.

Other Format:

Printed edition:

ISBN:

978-1-0716-1605-5

9781071616055

Access Restriction:

Restricted for use by site license.