Imaging and Quantifying Neuronal Autophagy / edited by Ben Loos, Esther Wong.

Format:

Book

Contributor:

Loos, Ben., Editor.

Wong, Esther, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Neuromethods 1940-6045 ; 171

Neuromethods, 1940-6045 ; 171

Language:

English

Subjects (All):

Neurosciences.

Neuroscience.

Local Subjects:

Neuroscience.

Physical Description:

1 online resource (XII, 150 pages) : 33 illustrations, 27 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker's yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques. .

Contents:

Quantification of Autophagosome Size and Formation Rate by Electron and Fluorescence Microscopy in Baker's Yeast

Ultrastructure of the Macroautophagy Pathway in Mammalian Cells

Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons

Monitoring Autophagic Activity In Vitro and In Vivo using the GFP-LC3-RFP-LC3∆G Probe

Measuring Autophagic Flux in Neurons by Optical Pulse Labeling

Measuring Autophagosome Flux

Measurement of Neuronal Tau Clearance In Vivo

Methods for Studying Axonal Autophagosome Dynamics in Adult Dorsal Root Ganglion Neurons

Imaging and Quantifying Neuronal Autophagy to Determine the Autophagy Contribution to Neuronal and Dendritic Morphogenesis

Correlative Light and Electron Microscopy (CLEM): Bringing Together the Best of Both Worlds to Study Neuronal Autophagy.

Other Format:

Printed edition:

ISBN:

978-1-0716-1589-8

9781071615898

Access Restriction:

Restricted for use by site license.