Therapeutic Antibodies : Methods and Protocols / edited by Gunnar Houen.

Format:

Book

Contributor:

Houen, Gunnar., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2313

Methods in Molecular Biology, 1940-6029 ; 2313

Language:

English

Subjects (All):

Biologicals.

Biopharmaceutics.

Biology-Technique.

Experimental immunology.

Pharmacology.

Biologics.

Immunological Techniques.

Local Subjects:

Biologics.

Immunological Techniques.

Pharmacology.

Physical Description:

1 online resource (XIII, 347 pages) : 71 illustrations, 61 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed book covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects. These biologics are the fastest growing pharmaceutical drug group and have had tremendous clinical and scientific impact in cancer, autoimmune diseases, infectious diseases, and other immune-related diseases, making the content of this volume essential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methods, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Antibodies: Methods and Protocols serves as an ideal guide for researchers working with the production of, research on, and development of therapeutic antibodies as well as for clinicians using therapeutic antibodies in daily work with patients.

Contents:

Therapeutic Antibodies: An Overview

Treatment of Autoimmune Diseases with Therapeutic Antibodies: Lessons Learned from PID Patients Allow for Stratification of the Infection Risk

B-Cell Analysis for Monitoring Patients Undergoing B-Cell Depletion for the Treatment of Autoimmune Diseases

Assessment of Therapeutic Antibody Developability by Combinations of In Vitro and In Silico Methods

The Therapeutic Antibody Profiler for Computational Developability Assessment

Short Read-Length Next-Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs

Large Scale Transient Production in ExpiCHO-STM with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection

Production of Therapeutic Single-Chain Variable Fragments (ScFv) in Pichia pastoris

Purification of Therapeutic Antibodies by Protein A Affinity Chromatography

Ion Exchange Chromatographic Methods for Purification of Therapeutic Antibodies

Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies

Use of PASEF for Accelerated Protein Sequence Confirmation and De Novo Sequencing with High Data Quality

Nano-Microscopy of Therapeutic Antibody Aggregates in Solution

Measuring Self-Association of Antibody Lead Candidates with Dynamic Light Scattering

Intact Mass Quantitation of Therapeutic Antibodies for Pharmacokinetic Studies Using Immuno-Purification

Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS

Bioassays for the Evaluation of Target Neutralization and Complement-Dependent Cytotoxicity (CDC) of Therapeutic Antibodies

Methods for Functional Characterization of FcRn Interactions with Therapeutic Antibodies and Fc-Fusion Proteins

Method for Measurement of Antibody-Dependent Cellular Phagocytosis

Bioluminescent Bridging Immunoassay for Anti-Drug Antibody (ADA) Detection

A Surface Plasmon Resonance-Based Assay for Simultaneous Measurement of Serum Concentrations of Therapeutic Antibodies and Anti-Drug Antibodies.

Other Format:

Printed edition:

ISBN:

978-1-0716-1450-1

9781071614501

Access Restriction:

Restricted for use by site license.