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RNAi Strategies for Pest Management : Methods and Protocols / edited by Luis María Vaschetto.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2360
- Methods in Molecular Biology, 1940-6029 ; 2360
- Language:
- English
- Subjects (All):
- Genetics.
- Agricultural genome mapping.
- Genetics and Genomics.
- Agricultural Genetics.
- Local Subjects:
- Genetics and Genomics.
- Agricultural Genetics.
- Physical Description:
- 1 online resource (XIV, 369 pages) : 63 illustrations, 49 illustrations in color.
- Edition:
- 1st ed. 2022.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2022.
- System Details:
- text file PDF
- Summary:
- This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems. Chapters in this book look at the design of novel RNAi-mediated pest control strategies and related issues. Some of the topics covered in this book are identification and characterization of differentially expressed non-coding RNAs in insect genomes; functional small RNA design; analysis of endogenous RNAi pathways; RNAi screening; plant models of RNAi-mediated pest control; and dsRNA delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNAi Strategies for Pest Management: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
- Contents:
- Genome-Wide Identification and Functional Characterization of Non-Coding RNAs (ncRNAs) Differentially Expressed during Insect Development
- Identification and Functional Characterization of Argonaute (Ago) Proteins in Insect Genomes
- Functional Characterization of Silkworm PIWI Proteins by Embryonic RNAi
- Guiding RNAi Design through Characterization of Endogenous Small RNA Pathways
- Silencing of Molecular Targets with Relevance to Insecticide Resistance in Colorado Potato Beetle using dsRNA
- Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In Situ Hybridization
- In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control
- RNAi Feeding Bioassay: A Protocol for dsRNA Screening against Asian Citrus Psyllid and Related Hemipteran Insects
- A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte)
- Rapid Screening of Myzus persicae (Green Peach Aphid) RNAi Targets using Tobacco rattle virus
- Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae
- Inhibition of Rhopalosiphum maidis (Corn Leaf Aphid) Growth on Maize by Virus-Induced Gene Silencing with Sugarcane mosaic virus
- RT-qPCR for Detection and Expression Monitoring of Core Genes Involved in the RNA Interference Pathways of Western Corn Rootworm (Diabrotica virgifera virgifera)
- Examination of the Suitability of Attractive Target Genes for RNAi-Based Pest Control
- Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peach Aphid, Myzus persicae
- Plant-Mediated RNA Interference Expressing dsRNA in Cytoplasm for RNAi-Based Pest Control
- RNAi Expression in Cotton for Control of Herbivorous Insects
- Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri
- Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115 (DE3) for Non-Transgenic RNAi-Based Insect Pest-Management
- Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects
- Application of Nanoparticle-Mediated RNAi for Efficient Gene Silencing and Pest Control on Soybean Aphids
- Methods for Delivery of dsRNAs for Agricultural Pest Control: The Case of Lepidopteran
- The CRISPR/Cas9-Mediated Genome Editing System in Insect Genomics and Pest Management.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-1633-8
- 9781071616338
- Access Restriction:
- Restricted for use by site license.
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