Invariant Natural Killer T-Cells : Methods and Protocols / edited by Chaohong Liu.

Format:

Book

Contributor:

Liu, Chaohong, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2388

Methods in Molecular Biology, 1940-6029 ; 2388

Language:

English

Subjects (All):

Lymphocytes.

Autoimmunity.

Immunology.

Local Subjects:

Lymphocytes.

Autoimmunity.

Immunology.

Physical Description:

1 online resource (XII, 195 pages) : 46 illustrations, 33 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Invariant Natural Killer T-Cells: Methods and Protocols is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system.

Contents:

Genetic Analysis of iNKT Cell Development and Function

Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells

Retroviral Transduction of NKT Hybridoma Cells

Methods for Studying Mouse and Human Invariant Natural Killer T-Cells

Isolation and Detection of Murine iNKT Cells in Different Organs

Identifying, Isolation, and Functional Use of Human Liver iNKT Cells

Isolation and Characterization Methods of Human Invariant NKT Cells

Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry

Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry

iNKT Cell Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach

Expansion of Human iNKT Cells Ex Vivo

The Expansion and Cytotoxicity Detection of Human iNKT Cells

Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood

Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic Mice

In Vivo Cytotoxicity by α-GalCer-Transactivated NK Cells

Redirecting iNKT Cell Anti-Tumor Immunity with αGalCer/CD1d-scFv Fusion Proteins

Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles during Development.

Other Format:

Printed edition:

ISBN:

978-1-0716-1775-5

9781071617755

Access Restriction:

Restricted for use by site license.