Circadian Regulation : Methods and Protocols / edited by Guiomar Solanas, Patrick -Simon Welz.

Format:

Book

Contributor:

Solanas, Guiomar, Editor.

Welz, Patrick-Simon, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2482

Methods in Molecular Biology, 1940-6029 ; 2482

Language:

English

Subjects (All):

Biochemistry.

Biology-Technique.

Biological Techniques.

Local Subjects:

Biochemistry.

Biological Techniques.

Physical Description:

1 online resource (XIV, 410 pages) : 84 illustrations, 74 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Circadian Regulation: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. Chapters 3, 4, 8, and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Sleep under Preindustrial Conditions: What We Can Learn from It

The structure-based molecular-docking screen against core clock proteins to identify small molecules to modulate the circadian clock

Analysis of complex circadian time series data using wavelets

Mathematical Modeling in Circadian Rhythmicity

Bioinformatics and Systems Biology of Circadian Rhythms: BIO CYCLE and CircadiOmics

Cell-based Phenotypic Screens to Discover Circadian Clock Modulating Compounds

Methods for Assessing Circadian Rhythms and Cell Cycle in Intestinal Enteroids

Using ALLIGATORs to capture circadian bioluminescence

Studying circadian clock entrainment by hormonal signals

In vitro assays for measuring intercellular coupling among peripheral circadian oscillators

Circadian control of transcriptional and metabolic rhythms in primary hepatocytes

Electrophysiology of the Suprachiasmatic Nucleus: Single-Unit Recording

Anatomical Methods to Study the Suprachiasmatic Nucleus

Circadian analysis of rodent locomotor activity in home cages

Recording of diurnal gene expression in peripheral organs of mice using the RT-Biolumicorder

Isolation and sorting of epidermal interfollicular stem cells for the study of circadian rhythms

Detecting circadian rhythms in human red blood cells by dielectrophoresis

Measuring circadian neutrophil infiltration in tissues by paired whole-mount tissue-clearing and flow cytometry

In vivo imaging of circadian NET formation during lung injury by 4-dimensional intravital microscopy

Real-time measurement of energy metabolism over circadian time using indirect calorimetry-enabled metabolic cages

Untargeted and Targeted Circadian Metabolomics Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Flow Injection-Electrospray Ionization-Tandem Mass Spectrometry (FIA-ESI-MS/MS)

Time Restricted Feeding and Caloric Restriction - two feeding regimen at the crossroad of metabolic and circadian regulation. -Chromatin Immunoprecipitation and Circadian Rhythms

Fluorescent reporters for studying circadian rhythms in Drosophila melanogaster

Visualization of mutant aggregates from clock neurons by agarose gel electrophoresis (AGERA) in Drosophila melanogaster

Methods for delivery of dsRNAi against canonical clock genes and immunocytodetection of clock proteins in crustacea

In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer. .

Other Format:

Printed edition:

ISBN:

978-1-0716-2249-0

9781071622490

Access Restriction:

Restricted for use by site license.