Proteomics in Systems Biology : Methods and Protocols / edited by Jennifer Geddes-McAlister.

Format:

Book

Contributor:

Geddes-McAlister, Jennifer, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2456

Methods in Molecular Biology, 1940-6029 ; 2456

Language:

English

Subjects (All):

Proteins.

Bioinformatics.

Computational and Systems Biology.

Local Subjects:

Proteins.

Computational and Systems Biology.

Physical Description:

1 online resource (XII, 370 pages) : 55 illustrations, 53 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed book highlights the diverse techniques and applications of proteomics in an accessible, informative, and concise manner. The collection features sample preparation from distinct extraction, quantification, enrichment, modification, as well as interactome methodology for the in-depth exploration of biological systems, and the application of proteomics to clinical, infectious disease, and agricultural practices. Moreover, cutting-edge bioinformatics tools, encompassing machine learning and data integration strategies, are explored, as are techniques expanding beyond proteomics into the realm of metabolomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting known pitfalls. Comprehensive and practical, Proteomics in Systems Biology: Methods and Protocols emphasizes the importance of proteomics and demonstrates a plethora of approaches for investigating diverse biological entities from a systems perspective.

Contents:

Review of the Real and Sometimes Hidden Costs in Proteomics Experimental Workflows

High-Throughput Mass Spectrometry-Based Proteomics with dia-PASEF

Isolation of Detergent Insoluble Proteins from Mouse Brain Tissue for Quantitative Analysis Using Data Independent Acquisition (DIA)

Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics

Protein Purification and Digestion Methods for Bacterial Proteomic Analyses

Mapping Cell Surface Proteolysis with Plasma Membrane-Targeted Subtiligase

N-Terminomics/TAILS of Tissue and Liquid Biopsies

HUNTER: Sensitive Automated Characterization of Proteolytic Systems by N Termini Enrichment from Microscale Specimen

Phosphoproteomics and Organelle Proteomics in Pancreatic Islets

Phosphoproteomic Sample Preparation for Global Phosphorylation Profiling of a Fungal Pathogen

Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes

Integrated Network Discovery Using Multi-Proteomic Data

Targeted Cross-Linking Mass Spectrometry on Single-Step Affinity Purified Molecular Complexes in the Yeast Saccharomyces cerevisiae

A Crosslinking - Mass Spectrometry Protocol for the Structural Analysis of Microtubule-Associated Proteins

Comprehensive Interactome Mapping of Nuclear Receptors Using Proximity Biotinylation

Mining Proteomics Datasets to Uncover Functional Pseudogenes

Proteomic Profiling of the Interplay between a Bacterial Pathogen and Host Uncovers Novel Anti-Virulence Strategies

Affinity-Enrichment of Salmonella-Modified Membranes from Murine Macrophages for Proteomic Analyses

Proteomic Profiling of Interplay between Agrobacterium tumefaciens and Nicotiana benthamiana for Improved Molecular Pharming Outcomes

Label-Free Quantitative Proteomic Profiling of Fusarium Head Blight in Wheat

DIA Proteomics and Machine Learning for the Fast Identification of Bacterial Species in Biological Samples

Novel Bioinformatics Strategies Driving Dynamic Metaproteomic Studies

MaxQuant Module for the Identification of Genomic Variants Propagated into Peptides

Untargeted Metabolomic Profiling of Fungal Species Populations.

Other Format:

Printed edition:

ISBN:

978-1-0716-2124-0

9781071621240

Access Restriction:

Restricted for use by site license.