Contemporary Approaches to the Study of Pain : From Molecules to Neural Networks / edited by Rebecca P. Seal.

Format:

Book

Contributor:

Seal, Rebecca P., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Neuromethods 1940-6045 ; 178

Neuromethods, 1940-6045 ; 178

Language:

English

Subjects (All):

Neurosciences.

Neuroscience.

Local Subjects:

Neuroscience.

Physical Description:

1 online resource (XIII, 458 pages) : 76 illustrations

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates; the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning. In the Neuromethods series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory. Cutting-edge and comprehensive, Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain.

Contents:

Single-Cell RNA-Sequencing of Somatosensory Neurons

Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis

Multiplex In Situ Hybridization of the Primate and Rodent DRG and Spinal Cord

Using Translating Ribosome Affinity Purification (TRAP) to Understand Cell-Specific Translatomes in Pain States

Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve

Single Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons using a Novel Ex Vivo Preparation

Electrophysiological Recording Techniques from Human Dorsal Root Ganglion

Human Pluripotent Stem Cell-Derived Sensory Neurons: A New Translational Approach to Study Mechanisms of Sensitization?

Intraspinal Transplantation of Precursors of Cortical GABAergic Interneurons to Treat Neuropathic Pain

A Co-Culture System for Studying Dorsal Spinal Cord Synaptogenesis

Visualizing Synaptic Connectivity using Confocal and Electron Microscopy: Neuroanatomical Approaches to Define Spinal Circuits

Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice

Recording Pain-Related Brain Activity in Behaving Animals using Calcium Imaging and Miniature Microscopes

Optical Imaging of the Spinal Cord for the Study of Pain: From Molecules to Neural Networks

In Vivo Calcium Imaging of Peripheral Ganglia

Optogenetic Modulation of the Visceromotor Response to Reveal Visceral Pain Mechanisms

Use of Optogenetics for the Study of Skin-Nerve Communication

Channelrhodopsin-2 Assisted Circuit Mapping in the Spinal Cord Dorsal Horn

Production of AAVs and Injection into the Spinal Cord

The Use of Intraspinally Injected Chemogenetic Receptor, PSAM-GlyR, to Probe the Functional Role of Spinal Dorsal Horn Neurons

Measuring Mouse Somatosensory Reflexive Behaviors with High-Speed Videography, Statistical Modeling, and Machine Learning.

Other Format:

Printed edition:

ISBN:

978-1-0716-2039-7

9781071620397

Access Restriction:

Restricted for use by site license.