Chromosome Architecture : Methods and Protocols / edited by Mark C. Leake.

Format:

Book

Contributor:

Leake, Mark C., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2476

Methods in Molecular Biology, 1940-6029 ; 2476

Language:

English

Subjects (All):

Chromosomes.

Local Subjects:

Chromosomes.

Physical Description:

1 online resource (XI, 330 pages) : 85 illustrations, 76 illustrations in color.

Edition:

2nd ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.

Contents:

A Next Generation of Advances in Chromosome Architecture

Single Molecule Narrowfield Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transduction of Live Yeast Cells

Convolutional Neural Networks for Classifying Chromatin Morphology in Live Cell Imaging

Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria

Atomic Force Microscopy of DNA and DNA-Protein Interactions

Live-Cell Visualization of DNA Transfer and Pilus Dynamics during Bacterial Conjugation

Transverse Magnetic Tweezers Allowing Coincident Epi-Fluorescence Microscopy on Horizontally Extended DNA

Atomistic Molecular Dynamics Simulations of DNA in Complex 3D Arrangements for Comparison with Lower Resolution Structural Experiments

Replication Labeling Methods for Super-Resolution Imaging of Chromosome Territories and Chromatin Domains

DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins

Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis

Dynamics of Bacterial Chromosomes by Locus Tracking in Fluorescence Microscopy

Measuring Nuclear Mechanics with Atomic Force Microscopy

Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells

Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells

Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy

The End Restraint Method for Mechanically Perturbing Nucleic Acids In Silico

Visualizing the Replisome, Chromosome Breaks, and Replication Restart in Bacillus subtilis

High-Throughput Imaging of Bacillus subtilis

Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis

Single-Molecular Quantification of Flowering Control Proteins within Nuclear Condensates in Live Whole Arabidopsis Root.

Other Format:

Printed edition:

ISBN:

978-1-0716-2221-6

9781071622216

Access Restriction:

Restricted for use by site license.