Cardiac Gene Therapy : Methods and Protocols / edited by Kiyotake Ishikawa.

Format:

Book

Contributor:

Ishikawa, Kiyotake, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2573

Methods in Molecular Biology, 1940-6029 ; 2573

Language:

English

Subjects (All):

Genetics.

Cardiology.

Medical genetics.

Medical Genetics.

Local Subjects:

Genetics.

Cardiology.

Medical Genetics.

Physical Description:

1 online resource (XIV, 347 pages) : 78 illustrations, 69 illustrations in color.

Edition:

2nd ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. .

Contents:

Updates on Cardiac Gene Therapy Research and Methods: Overview of Cardiac Gene Therapy

Tough Decoy-Mediated Cardiac Gene Suppression

Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes using miRcombo

CRISPR/Cas9 Gene Editing of RYR2 in Human iPSC Derived Cardiomyocytes to Probe Ca2+ Signaling Aberrancies of CPVT Arrhythmogenesis

Enhancing Cardiomyocyte Transcription using In Vivo CRISPR/Cas9 Systems

AAV Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model

Optimization of Synthesis of Modified mRNA

Design and Production of Heart Chamber-Specific AAV9 Vectors

Generation of Atrial-Specific Construct using Sarcolipin Promoter Associated CRM4 Enhancer

Cardiac Targeted Adeno-Associated Virus Injection in Rats

Cardiac Gene Delivery in Large Animal Models-Antegrade Techniques

Locked Nucleic Acid AntimiR Therapy for the Heart

Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection

Endocardial Gene Delivery using NOGA Catheter System

Surgical Methods for Cardiac Gene Delivery in Large Animals

Atrial Gene Painting in Large Animal Model of Atrial Fibrillation

Stent-Based Gene Delivery for Coronary Disease

Selective Anti-AAV Antibody Depletion by Hemapheresis and Immunoadsorption

Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy

Intra-Airway Gene Delivery for Pulmonary Hypertension in Rodent Models

Endo-Bronchial Gene Delivery for Pulmonary Hypertension in a Large Animal Model

Cell-Based Determination of Neutralizing Antibodies against Adeno-Associated Virus in Cardiac Gene Therapy

Left Ventricular Pressure Volume Assessment Using Carotid Artery Access in the Rat

Assessing the Effect of Cardiac Gene Therapy using Catheter-Based Pressure-Volume Measurement in Large Animals

ELIspot Assay for Gene Therapy in Large Animal Studies

Assessing Recombinant AAV Shedding after Cardiac Gene Therapy.

Other Format:

Printed edition:

ISBN:

978-1-0716-2707-5

9781071627075

Access Restriction:

Restricted for use by site license.