Neural Reprogramming : Methods and Protocols / edited by Henrik Ahlenius.

Format:

Book

Contributor:

Ahlenius, Henrik, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2352

Methods in Molecular Biology, 1940-6029 ; 2352

Language:

English

Subjects (All):

Neurosciences.

Biomedical engineering.

Neuroscience.

Biomedical Engineering and Bioengineering.

Local Subjects:

Neuroscience.

Biomedical Engineering and Bioengineering.

Physical Description:

1 online resource (XI, 263 pages) : 46 illustrations, 43 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research.

Contents:

Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts

Direct In Vitro Reprogramming of Astrocytes into Induced Neurons

Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9

Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules

Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons

Direct Conversion of Human Fibroblasts to Induced Neurons

Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts

Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs)

Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors

Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons

Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression

Transcriptional Profiling During Neural Conversion

Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo

Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods

Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions

CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders

Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells.

Other Format:

Printed edition:

ISBN:

978-1-0716-1601-7

9781071616017

Access Restriction:

Restricted for use by site license.