SARS-CoV-2 : Methods and Protocols / edited by Justin Jang Hann Chu, Bintou Ahmadou Ahidjo, Chee Keng Mok.

Format:

Book

Contributor:

Chu, Justin Jang Hann, Editor.

Ahidjo, Bintou Ahmadou, Editor.

Mok, Chee Keng, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2452

Methods in Molecular Biology, 1940-6029 ; 2452

Language:

English

Subjects (All):

Virology.

Local Subjects:

Virology.

Physical Description:

1 online resource (XVI, 478 pages) : 76 illustrations, 64 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.

Contents:

Evolution and Epidemiology of SARS-CoV-2 Virus

Molecular Epidemiology of SARS-CoV-2 by Sequencing

Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2

Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance

Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens

Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay

Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans

Propagation and Quantification of SARS-CoV-2

Quantification of Infectious SARS‐CoV‐2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay

One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics

Detection of SARS-CoV-2 by Mass Spectrometry

Bioinformatics-Based Approaches to Study Virus-Host Interactions During SARS-CoV-2 Infection

Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2

Animal Models of COVID-19: Nonhuman Primates

Animal Models of COVID-19: Transgenic Mouse Model

Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and Multiple Immunohistochemistry

Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses

Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach

Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test

Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2

Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2

Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2

Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility

Methods of SARS-CoV-2 Inactivation.

Other Format:

Printed edition:

ISBN:

978-1-0716-2111-0

9781071621110

Access Restriction:

Restricted for use by site license.