Reactive Oxygen Species in Plants : Methods and Protocols / edited by Amna Mhamdi.

Format:

Book

Contributor:

Mhamdi, Amna, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2526

Methods in Molecular Biology, 1940-6029 ; 2526

Language:

English

Subjects (All):

Botanical chemistry.

Plant physiology.

Stress (Physiology).

Plants.

Plant Biochemistry.

Plant Physiology.

Plant Stress Responses.

Local Subjects:

Plant Biochemistry.

Plant Physiology.

Plant Stress Responses.

Physical Description:

1 online resource (XIV, 298 pages) : 53 illustrations, 36 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.

Contents:

Modification of Chloroplast Antioxidant Capacity by Plastid Transformation

Analysis of Ascorbate Metabolism in Arabidopsis under High-Light-Stress

Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice

Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging

Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants

Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry

Measurement of NAD(P)H and NADPH-Generating Enzymes

Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells

Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe

A Simplified Method to Assay Protein Carbonylation by Spectrophotometry

In Vitro Biochemical Analysis of Recombinant Plant Proteins under Oxidation

Methods to Analyze the Redox Reactivity of Plant Proteins

Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase HPLC-MS under Non-Derivatized Conditions

Determination of Reactive Carbonyl Species, which Mediate Reactive Oxygen Species Signals in Plant Cells

Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds

Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM)

Quantitative Analysis of Post-Translational Modifications of Plant Histones

Characterization of RBPome in Oxidative Stress Conditions

Analysis of ROS-Triggered Changes in the Transcriptome.

Other Format:

Printed edition:

ISBN:

978-1-0716-2469-2

9781071624692

Access Restriction:

Restricted for use by site license.