Candida auris : Methods and Protocols / edited by Alexander Lorenz.

Format:

Book

Contributor:

Lorenz, Alexander, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2517

Methods in Molecular Biology, 1940-6029 ; 2517

Language:

English

Subjects (All):

Microbiology.

Fungi.

Mycology.

Local Subjects:

Microbiology.

Fungi.

Physical Description:

1 online resource (XV, 342 pages) : 76 illustrations, 60 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume covers the latest experimental protocols used by researchers to study the recently emerged fungal pathogen of humans, Candida auris, and also explores the pathogen's antifungal resistance, virulence, and pathogenicity. The chapters in this book cover topics such as the identification of C. auris in clinic samples by PCR and mass spectrometry; molecular typing to identify clade affiliation; genetic manipulation; genome analysis by bioinformatics; and phenotypic characterization using various biochemical, mass spectrometry, microscopy, and flow cytometry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Candida auris: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field.

Contents:

Isolation of Candida auris in Clinical Specimens

Candida auris Identification and Profiling by MALDI-ToF Mass Spectrometry

Molecular Tools for Candida auris Identification and Typing

qPCR Detection of Candida auris using the GPS™ CanAur MONODOSE dtec-qPCR Test

Susceptibility Testing of Candida auris Isolates

Deciphering Candida auris Paradoxical Growth Effect (Eagle Effect) in Response to Echinocandins

An Optimized Electroporation Procedure for Genetic Transformation of Candida auris

Genetic Transformation of Candida auris via Homology-Directed Repair Using a Standard Lithium-Acetate Protocol

Downregulation of Essential Genes in the Fungal Pathogen Candida auris

Cell Viability Assays for Candida auris

Measuring Gene Expression via mRNA Abundance in Candida auris

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Candida auris Metabolites

Isolation of Extracellular Vesicles from Candida auris

Labelling of Candida auris Cell Walls to Examine Levels of PAMP Exposure by Flow Cytometry and Fluorescence Microscopy

High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall

Generating Complete Genome Assemblies of Candida auris

MycoSNP: A Portable Workflow for Performing Whole-Genome Sequencing Analysis of Candida auris

Bioinformatic Identification of ABC Transporters in Candida auris

Examining Neutrophil-Candida auris Interactions with Human Neutrophils Ex Vivo

Modeling Candida auris Colonization on Porcine Skin Ex Vivo

A Human Ex Vivo Skin Model to Study Candida auris Biofilms

In Vivo Skin Colonization/Decolonization Models for Candida auris

Use of Galleria mellonella Larvae to Study the Pathogenicity and Clonal Lineage-Specific Behaviors of the Emerging Fungal Pathogen Candida auris

Drosophila melanogaster as a Rapid and Reliable In Vivo Infection Model to Study the Emerging Yeast Pathogen Candida auris

An Immunocompromised Mouse Model of Candida auris Systemic Infection

Mouse Gastrointestinal Colonization Model for Candida auris.

Other Format:

Printed edition:

ISBN:

978-1-0716-2417-3

9781071624173

Access Restriction:

Restricted for use by site license.