Catharanthus roseus : Methods and Protocols / edited by Vincent Courdavault, Sebastien Besseau.

Format:

Book

Contributor:

Courdavault, Vincent., Editor.

Besseau, Sebastien., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2505

Methods in Molecular Biology, 1940-6029 ; 2505

Language:

English

Subjects (All):

Botany.

Plant genetics.

Plant Science.

Plant Genetics.

Local Subjects:

Plant Science.

Plant Genetics.

Physical Description:

1 online resource (XIV, 319 pages) : 69 illustrations, 59 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms.

Contents:

RNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms

Imaging MS analysis in Catharanthus roseus

Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle

Sample preparation, data acquisition, and data analysis for 15N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus

Studying iridoid transport in Catharanthus roseus by grafting

Pictet-Spengler reaction for the chemical synthesis of strictosidine

Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids

Semi-Synthesis of Bis-Indole Alkaloid (-)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis

RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus

Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks

Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis

Ancestral sequence reconstruction for exploring alkaloid evolution

Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus

TARGETing transcriptional regulation in the medicinal plant Catharanthus roseus

Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

From Methylome to Integrative Analysis of Tissue-Specificity

Tagging and capture of prenylated CaaX-proteins from plant cell cultures

EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings

A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus

Transient gene expression in Catharanthus roseus flower petals using agroinfiltration

De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants

Agrobacterium-mediated in planta transformation in Periwinkle.

Other Format:

Printed edition:

ISBN:

978-1-0716-2349-7

9781071623497

Access Restriction:

Restricted for use by site license.