Histone Methyltransferases : Methods and Protocols / edited by Raphaël Margueron, Daniel Holoch.

Format:

Book

Contributor:

Margueron, Raphael, Editor.

Holoch, Daniel, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2529

Methods in Molecular Biology, 1940-6029 ; 2529

Language:

English

Subjects (All):

Enzymology.

Biology-Technique.

Gene expression.

Epigenetics.

Gene Expression Analysis.

Local Subjects:

Enzymology.

Gene Expression Analysis.

Epigenetics.

Physical Description:

1 online resource (XVII, 495 pages) : 72 illustrations, 53 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Contents:

Not all is SET for methylation: Evolution of eukaryotic protein methyltransferases

Detection and quantification of histone methyltransferase activity in vitro

In Vitro Histone Demethylase Assays

Preparation and characterization of chromatin templates for histone methylation assays

Techniques to study automethylation of histone methyltransferases and its functional impact

Rodriguez Profiling the regulation of histone methylation and demethylation by metabolites and metals

Determination of histone methyltransferase structure by crystallography

Determination of histone methyltransferase structures in complex with the nucleosome by cryogenic electron microscopy

Development and validation of antibodies targeting site-specific histone methylation

Genetic, genomic and imaging approaches to dissect the role of Polycomb group epigenetic regulators in mice

Profiling histone methylation in low numbers of cells

Automated CUT&RUN using the KingFisher Duo Prime

Bioinformatics methods for ChIP-seq histone analysis

Characterization of SET-domain histone lysine methyltransferase substrates using a cofactor S-adenosyl-L-methionine surrogate

Specificity analysis of protein methyltransferases and discovery of novel substrates using SPOT peptide arrays

Identifying specific protein interactors of nucleosomes carrying methylated histones using mass spectrometr

Investigating mitotic inheritance of histone post-translational modifications by triple pSILAC coupled to Nascent Chromatin Capture

Investigating mitotic inheritance of histone modifications using tethering

Investigating histone modification dynamics by mechanistic computational modelling

Screening for small molecule inhibitors of histone methyltransferases.

Other Format:

Printed edition:

ISBN:

978-1-0716-2481-4

9781071624814

Access Restriction:

Restricted for use by site license.