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Plant Circular RNAs : Methods and Protocols / edited by Luis María Vaschetto.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2362
- Methods in Molecular Biology, 1940-6029 ; 2362
- Language:
- English
- Subjects (All):
- Plant genetics.
- Genetics.
- Bioinformatics.
- Plant Genetics.
- Genetics and Genomics.
- Local Subjects:
- Plant Genetics.
- Genetics and Genomics.
- Bioinformatics.
- Physical Description:
- 1 online resource (X, 196 pages) : 43 illustrations
- Edition:
- 1st ed. 2021.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2021.
- System Details:
- text file PDF
- Summary:
- This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs' research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field. .
- Contents:
- Identification and Characterization of Plant Circular RNAs
- Experimental Strategies for Studying the Function of Plant circRNAs
- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization
- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development
- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing
- Identification and Functional Characterization of Viroid Circular RNAs
- Circular RNA Databases
- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs
- Computational Analysis of Transposable Elements and circRNAs in Plants
- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database
- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-1645-1
- 9781071616451
- Access Restriction:
- Restricted for use by site license.
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