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piRNA : Methods and Protocols / edited by Nicholas F. Parrish, Yuka W. Iwasaki.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2509
- Methods in Molecular Biology, 1940-6029 ; 2509
- Language:
- English
- Subjects (All):
- Biomaterials.
- Nucleic acids.
- Proteins.
- Nucleic Acid.
- Protein Biochemistry.
- Local Subjects:
- Nucleic Acid.
- Protein Biochemistry.
- Physical Description:
- 1 online resource (XII, 397 pages) : 83 illustrations, 73 illustrations in color.
- Edition:
- 1st ed. 2022.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2022.
- System Details:
- text file PDF
- Summary:
- This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, piRNA: Methods and Protocols serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology.
- Contents:
- Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides
- CRISPR-Mediated Genome Engineering in Aedes aegypti
- PIWI-Directed DNA Elimination for Tetrahymena Genetics
- Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing
- Isolation and Processing of Bovine Oocytes for Small RNA Sequencing
- 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther
- Cloning, Sequencing, and Linkage Analysis of piRNAs
- Drosophila Genetic Resources for Elucidating piRNA Pathway
- Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System
- Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline
- CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells
- Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability
- Revisiting the Glass Treatment for Single Molecule Analysis of ncRNA Function
- Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification
- Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq
- UPA-Seq-Based Search Method for Functional lncRNA Candidates
- Large-Scale Analysis of RNA-Protein Interactions for Functional RNA Motif Discovery Using FOREST
- Computational Methods for the Discovery and Annotation of Viral Integrations
- Bioinformatics Approaches for Determining the Functional Impact of Repetitive Elements on Non-Coding RNAs
- Extending and Running the Mosquito Small RNA Genomics Resource Pipeline
- Preparation of Non-Overlapping Transposable Elements (TE) Annotation by Interval Tree
- Statistical Thermodynamics Approach for Intracellular Phase Separation.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-2380-0
- 9781071623800
- Access Restriction:
- Restricted for use by site license.
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