Mycobacterium ulcerans : Methods and Protocols / edited by Gerd Pluschke, Katharina Röltgen.

Format:

Book

Contributor:

Pluschke, Gerd., Editor.

Röltgen, Katharina, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2387

Methods in Molecular Biology, 1940-6029 ; 2387

Language:

English

Subjects (All):

Microbiology.

Immunology.

Pathology.

Local Subjects:

Microbiology.

Immunology.

Pathology.

Physical Description:

1 online resource (X, 232 pages) : 33 illustrations, 25 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.

Contents:

Overview: Mycobacterium ulcerans Disease (Buruli ulcer)

Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin Tissue

Primary Isolation of Mycobacterium ulcerans

Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance

Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture

DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR

Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans

Detection of Mycobacterium ulcerans DNA using Real-Time PCR

Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR

Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future Directions

Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulcerans

Conditions for Handling and Optimal Storage of Mycolactone

Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples

Detection of Mycolactone by Thin Layer Chromatography

Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone

Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61

Overview: Development of Drugs against Mycobacterium ulcerans

In Vitro Assessment of Drug Activity against Mycobacterium ulcerans

Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcer

Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans

Determination of Bioenergetic Parameters in Mycobacterium ulcerans.

Other Format:

Printed edition:

ISBN:

978-1-0716-1779-3

9781071617793

Access Restriction:

Restricted for use by site license.