Plant Circadian Networks : Methods and Protocols / edited by Dorothee Staiger, Seth Davis, Amanda Melaragno Davis.

Format:

Book

Contributor:

Staiger, Dorothee, Editor.

Davis, Seth, Editor.

Davis, Amanda Melaragno, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2398

Methods in Molecular Biology, 1940-6029 ; 2398

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XI, 245 pages) : 50 illustrations, 33 illustrations in color.

Edition:

2nd ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Plant Circadian Networks: Methods and Protocols, Second Edition is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general. "Period estimation and rhythm detection in timeseries data using BioDare2, the free, online, community resource" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Circadian Rhythm: Phase Response Curve and Light Entrainment

Period Estimation and Rhythm Detection in Timeseries Data Using BioDare2, the Free, Online, Community Resource

Detection and Analysis of Circadian Rhythms Via Prompt Chlorophyll Fluorescence

High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock

Agrobacterium-mediated seedling transformation to measure circadian rhythms in Arabidopsis

Measurement of Luciferase Rhythms in Soybean Hairy Roots.-ODE (ordinary differential equation) Models for Plant Circadian Networks: What The Models Are, and How They Should Be Used

How to Detect QTLs in the Plant Circadian Clock

Measuring Hypocotyl Length in Arabidopsis

High Throughput Extraction and Enzymatic Determination of Sugars and Fructans In Fructan-Accumulating Plants

Simple Nuclei Preparation and Co-Immunoprecipitation Procedures for Studying Protein Abundance and Interactions In Plant Circadian Time Courses

Chromatin Immunoprecipitation Protocol for Circadian Clock Proteins

A high-throughput 3'-Tag RNA Sequencing for Large-Scale Time-Series Transcriptome Studies

Experimental Design for Time-Series RNA-seq Analysis of Gene Expression and Alternative Splicing

Using tandem affinity purification to identify circadian clock protein complexes from Arabidopsis

Firefly Luciferase Complementation-Based Analysis of Dynamic Protein-Protein Interactions under Diurnal and Circadian Conditions in Arabidopsis

Monitoring Seasonal Bud Set, Bud Burst, and Cold Hardiness in Populus

The Perennial Clock Is an Essential Timer for Seasonal Growth Events and Cold Hardiness.

Other Format:

Printed edition:

ISBN:

978-1-0716-1912-4

9781071619124

Access Restriction:

Restricted for use by site license.