Post-Transcriptional Gene Regulation / edited by Erik Dassi.

Format:

Book

Contributor:

Dassi, Erik, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2404

Methods in Molecular Biology, 1940-6029 ; 2404

Language:

English

Subjects (All):

Biology-Technique.

Gene expression.

Genetic transcription.

Gene Expression Analysis.

Gene Transcription.

Local Subjects:

Gene Expression Analysis.

Gene Transcription.

Physical Description:

1 online resource (XIV, 411 pages) : 71 illustrations, 64 illustrations in color.

Edition:

3rd ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA modifications, the book guides molecular biologists toward harnessing the power of this new generation of techniques, while also introducing the data analysis skills that these high-throughput techniques require. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Post-Transcriptional Gene Regulation, Third Edition serves as a versatile resource for researchers studying post-transcriptional regulation by both introducing the most recent techniques and providing a comprehensive guide to their implementation. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression

Predicting RNA Secondary Structure Using In Vitro and In Vivo Data

RBPmap: A Tool for Mapping and Predicting the Binding Sites of RNA-Binding Proteins Considering the Motif Environment

Analysis of mRNA Translation by Polysome Profiling

Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling

Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq

Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems

Studying RNP Composition with RIP

PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites

A Pipeline for Analyzing eCLIP and iCLIP Data with htseq-clip and DEWSeq

Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH)

Identifying the Protein Interactomes of Target RNAs Using HyPR-MS

Visualization and Quantification of Subcellular RNA Localization Using Single Molecule RNA Fluorescence In Situ Hybridization

Single-Molecule RNA Imaging Using Mango II Arrays

Genome-Wide Identification of Polyadenylation Dynamics with TED-Seq

In Vivo RNA Structure Probing with DMS-MaPseq

Transcriptome-Wide Profiling of RNA Stability

High-Throughput Quantitation of Yeast uORF Regulatory Impacts Using FACS-uORF

m6A RNA Immunoprecipitation Followed by High-Throughput Sequencing to Map N6-Methyladenosine

Detecting m6A with In Vitro DART-Seq

Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing

Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.

Other Format:

Printed edition:

ISBN:

978-1-0716-1851-6

9781071618516

Access Restriction:

Restricted for use by site license.