Cell Penetrating Peptides : Methods and Protocols / edited by Ülo Langel.

Format:

Book

Contributor:

Langel, Ülo, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2383

Methods in Molecular Biology, 1940-6029 ; 2383

Language:

English

Subjects (All):

Proteins.

Cytology.

Cell Biology.

Local Subjects:

Proteins.

Cell Biology.

Physical Description:

1 online resource (XX, 632 pages) : 103 illustrations, 89 illustrations in color.

Edition:

3rd ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This third edition summarizes the most important areas of Cell-Penetrating Peptides (CPP) research. Chapters are divided into three parts detailing the historical background of CPP, the classifications of the available CPPs, approaches for prediction of novel CPPs, methods for studies of "naked" CPPs, and a brief summary of functionality issues of CPPs, both in vitro and in vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cell Penetrating Peptides: Methods and Protocols, Third Edition aims to be a useful practical guide to researches to help further their study in this field.

Contents:

Cell-penetrating peptides

Shuttling homeoproteins and their biological significance

Artificial evolutionary optimization process to improve the functionality of CPPs

In silico design of chemically modified cell penetrating peptides

Synthetic Molecular Evolution of Cell Penetrating Peptides

A native CPP from rattlesnake with therapeutic and theranostic properties

Synthesis of cell-penetrating peptide coated silica nanoparticles and their physicochemical and biological characterization

Enhancing the therapeutic potential of extracellular vesicles using peptide technology

Challenges and methods for the study of CPP translocation mechanisms

Membrane molecular interactions and induced structures of CPPs

A single GUV method for revealing the action of cell-penetrating peptides in biomembranes

Methods for Small-Angle Scattering Measurements on Peptiplexes of DNA with Cell-Penetrating Peptides

Imaging CPP delivery mechanisms of oligonucleotides

Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in non-adherent cells

PepFect14: signaling and transfection

Utilisation of cell-penetrating peptides for in vivo delivery of bioactive cargo - the effect of nanoparticle formulation

Protein delivery by PTDs/CPPs

Protein Delivery to Cytosol by Cell-Penetrating Peptide Bearing a Tandem Repeat Penetration-Accelerating Sequence

Recombinant transcription factors (TFs) fused to ZEBRA minimal transduction domain (MD) for modulation of mRNA transcripts

Protein mimicry and the design of bioactive cell penetrating peptides: The genesis of STOPSPERM bioportides

Lipopeptide pepducins as therapeutic agents

Delivery of Antibiotics by Cell-Penetrating Peptides to Kill Intracellular Pathogens

CPP applications in immune modulation and disease therapy

Cell-penetrating peptides as carriers for transepithelial drug delivery

New tools for streamlined in vivo homing peptide identification

Tumor targeting as new therapeutic approach

Mitochondrial Targeting Probes, drug conjugates and gene therapeutics

Novel brain penetrating single chain antibodies directed against 3RTau for the Treatment of Alzheimer's Disease and related Dementias

Strategies for peptide-mediated cargo delivery to human smooth muscle cells

Tips and Tools to Understand Direct Membrane Translocation of siRNA-Loaded WRAP-Based Nanoparticles

Evaluating efficacy of peptide-delivered oligonucleotides using the severe Taiwanese SMA mouse model

Development of CPP-based methods for delivery of miRNAs into the skin and airways - lessons from cell culture and mouse models

Endpoint and kinetic approaches for assessing transfection efficacy in mammalian cell culture

Tissue analysis of lung-targeted delivery of siRNA and plasmid DNA

Improvement of transfection with PepFects by organic and inorganic components

DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent

Use of PepFect14 and siRNA for targeted gene silencing in human embryonic stem cells

CRISPR/Cas9 plasmid delivery through the CPP: PepFect14

Applications of CPPs in genome editing of plants

Live Cell Genomics: Cell-specific transcriptome capture and exon-specific RNA binding protein isolation in live tissues and cells.

Other Format:

Printed edition:

ISBN:

978-1-0716-1752-6

9781071617526

Access Restriction:

Restricted for use by site license.