Bacterial Vaccines : Methods and Protocols / edited by Fadil Bidmos, Janine Bossé, Paul Langford.

Format:

Book

Contributor:

Bidmos, Fadil, Editor.

Bosse, Janine, Editor.

Langford, Paul, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2414

Methods in Molecular Biology, 1940-6029 ; 2414

Language:

English

Subjects (All):

Bacteria.

Virology.

Local Subjects:

Bacteria.

Virology.

Physical Description:

1 online resource (XII, 453 pages) : 110 illustrations, 89 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

The importance of vaccines to combat bacterial diseases cannot be overstated. Methods used in the development and testing of these vaccines are evolving rapidly as a direct consequence of the availability of advanced technologies. This volume will cover methods developed in the last decade, the usage of which are enabling the development of cheaper, cost-effective and structurally stable vaccines for global use. Chapters cover in silico analytical methods such as reverse vaccinology and machine learning; low-energy electron irradiation for the generation of inactivated bacterial vaccines; methods for assessment of OMV/GMMA quality and stability; and controlled human infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Bacterial Vaccines: Methods and Protocols is a valuable resource for novice and expert researchers interested in learning more about this important and constantly evolving field. .

Contents:

Vaccine Design by Reverse Vaccinology and Machine Learning

Application of Reverse Vaccinology and Immunoinformatic Strategies for the Identification of Vaccine Candidates against Shigella flexneri

Purification of Prospective Vaccine Antigens from Gram-Positive Pathogens by Immunoprecipitation

Rapid Surface Shaving for Proteomic Identification of Novel Surface Antigens for Vaccine Development

Two-Dimensional Electrophoresis Coupled with Western Blot as a Method to Detect Potential Neutralizing Antibody Targets from Gram-Negative Intracellular Bacteria

Panproteome Analysis of the Human Antibody Response to Bacterial Vaccines and Challenge

Low-Energy Electron Irradiation (LEEI) for the Generation of Inactivated Bacterial Vaccines

Design and Production of Hybrid Antigens for Targeting Integral Outer Membrane Proteins in Gram-Negative Bacteria

Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit Vaccine Delivery

Multiepitope Fusion Antigen - MEFA, An Epitope- and Structure-Based Vaccinology Platform for Multivalent Vaccine Development

Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development

Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures

Glycine Induction Method: Effective Production of Immunoactive Bacterial Membrane Vesicles with Low Endotoxin Content

Methods for Assessment of OMV/GMMA Quality and Stability

Production of Vaccines using Biological Conjugation

Immunological Assessment of Lung Responses to Inhalational Lipoprotein Vaccines against Bacterial Pathogens

Determination of Maternal and Infant Immune Responses to Pertussis Vaccination in Pregnancy

Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma

Assessment of Serum Bactericidal and Opsonophagocytic Activity of Antibodies to Gonococcal Vaccine Targets

Opsonophagocytic Killing Assay to Measure Anti-Group A Streptococcus Antibody Functionality in Human Serum

Neisseria lactamica Controlled Human Infection Model

Analyzing Macrophage Infection at the Organ Level

Multi-Color Flow Cytometry and High-Dimensional Data Analysis to Probe Complex Questions in Vaccinology.

Other Format:

Printed edition:

ISBN:

978-1-0716-1900-1

9781071619001

Access Restriction:

Restricted for use by site license.