Viroids : Methods and Protocols / edited by Ayala L. N. Rao, Irene Lavagi-Craddock, Georgios Vidalakis.

Format:

Book

Contributor:

Rao, Ayala L. N., Editor.

Lavagi-Craddock, Irene, Editor.

Vidalakis, Georgios, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2316

Methods in Molecular Biology, 1940-6029 ; 2316

Language:

English

Subjects (All):

Plant genetics.

Plant Genetics.

Local Subjects:

Plant Genetics.

Physical Description:

1 online resource (XIII, 375 pages) : 81 illustrations, 48 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Viroids: Methods and Protocols is a valuable resource for researchers and graduate students who are working with viroid diseases. .

Contents:

Study and Detection of Citrus Viroids in Woody Hosts

Detection and Characterization of Viroids via Biological Assays on Herbaceous Hosts

Analysis on RNA Motif-Based RNA Trafficking in Plants

Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis

High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroids

Extraction and Purification of Viroids from Herbaceous Hosts

Detection of Viroids by sPAGE Gel Electrophoresis

Two-Dimensional Polyacrylamide-Gel Electrophoresis Analysis of Viroid RNAs

A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae

Gel Blot Hybridization for Viroids

Tissue Print Hybridization for Detection and Characterization of Viroids

Fluorescein-Based Electrophoretic Mobility Shift Assay

Detection of Viroids by RT-PCR

Detection of Viroids using RT-qPCR

Multiplex RT-PCR

Real-Time Detection of Viroids using Singleplex and Multiplex Quantitative Polymerase Chain Reaction

Reverse Transcription-Loop Mediated Isothermal AMPlification (RT-LAMP) at the Service of Viroid Detection

SYBR ® Green RT-qPCR for the Universal Detection of Citrus Viroids

Detection of Avocado sunblotch and Other Viroids using RNA Filter Paper Capture and RT-PCR

Cloning and Sequencing of Viroids

QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification

Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms

An In Silico Detection of a Citrus Viroid from Raw High Throughput Sequencing Data

Detection of Viroid RNA and vd-siRNA in N. benthamiana Plants: Northern Blot Analyses for Viroid and vd-siRNAs

Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold

Predicting the Structure of a Viroid.

Other Format:

Printed edition:

ISBN:

978-1-0716-1464-8

9781071614648

Access Restriction:

Restricted for use by site license.