Craniofacial Development : Methods and Protocols / edited by Sebastian Dworkin.

Format:

Book

Contributor:

Dworkin, Sebastian, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2403

Methods in Molecular Biology, 1940-6029 ; 2403

Language:

English

Subjects (All):

Developmental biology.

Cytology.

Developmental Biology and Stem Cells.

Cell Biology.

Local Subjects:

Developmental Biology and Stem Cells.

Cell Biology.

Physical Description:

1 online resource (XII, 313 pages) : 94 illustrations, 73 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. .

Contents:

1. Using Caenorhabditis elegans as a model for mechanistic insights of craniofacial development

Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial Developmen

Elucidationof Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-MountIn Situ Hybridization

Visualizationof the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining

Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue

Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion

Single cell RNA-seq: Cell Isolation and Data Analysis

Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9

Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures

Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull

Characterization of mammalian in vivo enhancers using mouse transgenesis and CRISPR genome editing

Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton

Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development

Ex Vivo Culture of Human Cranial Suture Cells

Scaffolds for Use in Craniofacial Bone Regeneration

Mandible Explant Assay for The Analysis of Meckel's Cartilage Development

High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models

Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration

Salivary Gland Development In Culture

Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis.

Other Format:

Printed edition:

ISBN:

978-1-0716-1847-9

9781071618479

Access Restriction:

Restricted for use by site license.