Mitosis : Methods and Protocols / edited by Edward H. Hinchcliffe.

Format:

Book

Contributor:

Hinchcliffe, Edward H., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2415

Methods in Molecular Biology, 1940-6029 ; 2415

Language:

English

Subjects (All):

Cell division.

Cytology.

Cell Division.

Cell Biology.

Local Subjects:

Cell Division.

Cell Biology.

Physical Description:

1 online resource (XI, 256 pages) : 55 illustrations, 44 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume collects a selection of key techniques for studying cell division, representing multiple model systems and varied scales of approach. Over the past 20 years, a series of revolutions in experimental molecular biology, including chimeric fluorescent protein expression, multiple advanced modes of quantitative microscopy, and array of small molecule inhibitors, proteomic profiling, and gene silencing/manipulation/analysis, has advanced the mitosis field to a point where single cell biology not only allows for imaging/localization studies, but also for quantitative analysis and sequencing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols provides a repository of techniques and approaches for those working in the field as well as a working resource for those venturing into the study of mitosis for the first time.

Contents:

Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis

Methods for Investigating Cell Division Mechanisms in C. elegans

Visualizing the Dynamics of Cell Division by Live Imaging Drosophila Larval Brain Squashes

Imaging Chambers for Arabidopsis Seedlings for Mitotic Studies

Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells

Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells

Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin

Using Microinjection of Mammalian Cultured Cells to Study Cell Division

In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments during Mitosis

Quantifying Changes in Chromosome Position to Assess Chromokinesin Activity

Chemical Biology of Mitotic Spindle Assembly Motors

Deploying Kinase Inhibitors to Study Pediatric Gliomas

Identifying Cyclin A/Cdk1 Substrates in Mitosis in Human Cells

Degron Tagging Using mAID and SMASh Tags in RPE-1 Cells

Centromere Tension Measurement in Budding Yeast Mitosis

Simulating Dynamic Chromosome Compaction: Methods for Bridging In Silico to In Vivo

Using FLIM-FRET for Characterizing Spatial Interactions in the Spindle

Imaging Mitosis with Lattice LightSheet.

Other Format:

Printed edition:

ISBN:

978-1-0716-1904-9

9781071619049

Access Restriction:

Restricted for use by site license.