The Unfolded Protein Response : Methods and Protocols / edited by Roberto Pérez-Torrado.

Format:

Book

Contributor:

Torrado, Roberto Pérez-, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2378

Methods in Molecular Biology, 1940-6029 ; 2378

Language:

English

Subjects (All):

Proteins.

Local Subjects:

Proteins.

Physical Description:

1 online resource (XII, 332 pages) : 64 illustrations, 53 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume is divided in six section covering the most experimental approaches involved in the study of the unfolded protein response (UPR) pathway. Chapters detail determination of unfolded protein levels, methods to study UPR signal transmission, analysing the outcomes of the UPR pathway activation, UPR studies in mammalian models, UPR in alternative models, and UPR and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Unfolded Protein Response: Methods and Protocols aims to describe key methods and approaches used in the study of the UPR pathway and its complex cellular implications. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Measuring cysteine exposure in unfolded proteins with tetraphenylethene maleimide and its analogues

Fluorescence-based biosensors for the detection of the unfolded protein response

Indirect methods to measure unfolded proteins in living cells using fluorescent proteins

A platform technology for monitoring the Unfolded Protein Response

The use of fluorescent protein fusions to monitor the unfolded protein response and protein foldase-substrate interactions in plant protoplasts

High-throughput analysis of protein turnover with tandem fluorescent protein timers

Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae

Determination of protein translation during the activation of the UPR

Determination of the stability and intracellular (intra-nuclear) targeting and recruitment of pre-HAC1 mRNA in the Saccharomyces cerevisiae during the activation of UPR

Assays to study IRE1 activation and signaling

Analysis of XBP1 contribution to hyperosmolarity-induced lipid synthesis

Integrating an ER stress reporter for monitoring genome wide UPR-ER in budding yeast

Analyze mouse knockout models of UPR pathway elements

In vitro stimulation of IRE1a/XBP1-deficient B cells with LPS

Detection of PERK signaling in the central nervous system

Methods to study the mitochondrial unfolded protein response (UPRmt) in Caenorhabditis elegans

Drosophila Unfolded Protein Response (UPR) assays in vitro and in vivo

Protein preparation for proteomic analysis of the unfolded protein response in Arabidopsis thaliana

Structure-based drug discovery of IRE1 modulators

Chemical screening to detect ER stress regulators.

Other Format:

Printed edition:

ISBN:

978-1-0716-1732-8

9781071617328

Access Restriction:

Restricted for use by site license.