HIV Reservoirs : Methods and Protocols / edited by Guido Poli, Elisa Vicenzi, Fabio Romerio.

Format:

Book

Contributor:

Poli, Guido, Editor.

Vicenzi, Elisa, Editor.

Romerio, Fabio, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2407

Methods in Molecular Biology, 1940-6029 ; 2407

Language:

English

Subjects (All):

Internal medicine.

Internal Medicine.

Local Subjects:

Internal Medicine.

Physical Description:

1 online resource (XIV, 451 pages) : 61 illustrations

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.

Contents:

Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency

U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency

An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latencyin primary human CD4+ T cells

The cultured TCM model of HIV latency

A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) Method

TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model.p Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoirs Quantification

Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF)

Human monocyte-derived macrophages (MDM) - Model 2

Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100

Hematopoietic stem and progenitor cells (HSPCs)

HIV reservoirs: Modeling, Quantification and Approaches to a Cure

More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication

Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells

Decidua basalis: An ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond

HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure

Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research

Inducing long-term HIV-1 latency in the TKO-BLT mouse model

In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue

Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH

Ex vivo differentiation of resting CD4+ T lymphocytes enhances detection of replication competent HIV-1 in viral outgrowth assays

Quantitative Viral Outgrowth Assay to Measure the functional SIV Reservoir in Myeloid Cells

Near-full-length single-genome HIV-1 DNA sequencing

TILDA: Tat/rev Induced Limiting Dilution Assay

Latency Reversal and Clearance of Persistent HIV Infection

Cure and long-term remission strategies

Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils for CRISPR Therapy. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1871-4

9781071618714

Access Restriction:

Restricted for use by site license.