Cell-Free Gene Expression : Methods and Protocols / edited by Ashty S. Karim, Michael C. Jewett.

Format:

Book

Contributor:

Karim, Ashty S., Editor.

Jewett, Michael C., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2433

Methods in Molecular Biology, 1940-6029 ; 2433

Language:

English

Subjects (All):

Synthetic biology.

Biology-Technique.

Gene expression.

Biotechnology.

Synthetic Biology.

Gene Expression Analysis.

Local Subjects:

Synthetic Biology.

Gene Expression Analysis.

Biotechnology.

Physical Description:

1 online resource (XV, 435 pages) : 80 illustrations, 63 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

Contents:

Best Practices for DNA Template Preparation towards Improved Reproducibility in Cell-Free Protein Production

Simple Extract Preparation Methods for E. coli-Based Cell-Free Expression

Preparation and Screening of Cell-Free Extract from Non-Growing Escherichia coli A19 Cells

Cell-Free Protein Synthesis Using Pichia pastoris

A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression

In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins

High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems

Cell-Free Gene Expression from DNA Brushes

Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications

Measurement of Transcription, Translation, and Other Enzymatic Processes during Cell-Free Expression Using PERSIA

Cell-Free Noncanonical Redox Cofactor Systems

Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping

Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry

Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression

Cell-Free Membrane Protein Expression into Hybrid Lipid/Polymer Vesicles

Cell-Free Synthesis Strategies to Probe Co-Translational Folding of Proteins within Lipid Membranes

Assembly of RNA Nanostructures from Double Crossover Tiles

Cell-Free Biosensors and AI Integration

ROSALIND: Rapid Detection of Chemical Contaminants with In Vitro Transcription Factor-Based Biosensors

Optical Sensing in Cell-Free Expression

Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers

Detection of Norovirus Using Paper-Based Cell-Free Systems

A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes

Implementing Hands-On Molecular and Synthetic Biology Education Using Cell-Free Technology.

Other Format:

Printed edition:

ISBN:

978-1-0716-1998-8

9781071619988

Access Restriction:

Restricted for use by site license.