Cancer Biomarkers : Methods and Protocols / edited by Gagan Deep.

Format:

Book

Contributor:

Deep, Gagan, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2413

Methods in Molecular Biology, 1940-6029 ; 2413

Language:

English

Subjects (All):

Tumor markers.

Cancer.

Tumour Biomarkers.

Cancer Biology.

Local Subjects:

Tumour Biomarkers.

Cancer Biology.

Physical Description:

1 online resource (XI, 258 pages) : 72 illustrations, 55 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume explores numerous methods used in basic science laboratories to characterize cancer-related biomarkers, vital for better managing cancer burden, including cancer risk assessment, cancer diagnosis, determining cancer progression, and therapeutic response. From a radiography method to an examination of single-cell RNA-seq and computational analysis tools in cancer research, this book delves into many techniques that could provide valuable molecular information about the tumor and its microenvironment components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cancer Biomarkers: Methods and Protocols offers researchers multiple helpful ways to study cancer-associated molecular biomarkers.

Contents:

A Method of Bone-Metastatic Tumor Progression Assessment in Mice Using Longitudinal Radiography

Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice

Method to Development of PET Radiopharmaceutical for Cancer Imaging

PET Use in Cancer Diagnosis, Treatment, and Prognosis

Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor

Zebrafish Xenograft Model to Study Human Cancer

Assessing Oligomerization Status of Mitochondrial OXPHOS Complexes via Blue Native Page

In Vitro Cell Impedance Assay to Examine Antigen-Specific T-Cell-Mediated Melanoma Cell Killing to Support Cancer Immunotherapy Drug Discovery

Methods to Detect Nitric Oxide and Reactive Nitrogen Species in Biological Sample

Study of Rotary Cell Culture System Induced Microgravity Effects on Cancer Biomarkers

A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons

Zymography and Reverse Zymography for Testing Proteases and Their Inhibitors

Method of Preparation of Cigarette Smoke Extract to Assess Lung Cancer-Associated Changes in Airway Epithelial Cells

Air-Liquid Interface Culture Model to Study Lung Cancer-Associated Cellular and Molecular Changes

Co-Immunoprecipitation-Blotting: Analysis of Protein-Protein Interactions

Methods to Assess Oxidative DNA Base Damage Repair of Apurinic/Apyrimidinic (AP) Sites Using Radioactive and Non-Radioactive Oligonucleotide-Based Assays

Determining the Size Distribution and Integrity of Extracellular Vesicles by Dynamic Light Scattering

Characterization of Exosomal Surface Proteins by Immunogold Labeling

Scanning Electron Microscopy of Giant Cells from Giant Cell Tumor of Bone

Raman Microscopy Techniques to Study Lipid Droplet Composition in Cancer Cells

Surface Plasmon Resonance, a Novel Technique for Sensing Cancer Biomarker: Folate Receptor and Nanoparticles Interface

Characterization of Tobacco Microbiome by Metagenomics Approach

Single-Cell RNA-Seq Technologies and Computational Analysis Tools: Application in Cancer Research.

Other Format:

Printed edition:

ISBN:

978-1-0716-1896-7

9781071618967

Access Restriction:

Restricted for use by site license.