Biomedical Engineering Technologies : Volume 2 / edited by Avraham Rasooly, Houston Baker, Miguel R. Ossandon.

Format:

Book

Contributor:

Rasooly, Avraham, Editor.

Baker, Houston., Editor.

Ossandon, Miguel R., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2394

Methods in Molecular Biology, 1940-6029 ; 2394

Language:

English

Subjects (All):

Biotechnology.

Local Subjects:

Biotechnology.

Physical Description:

1 online resource (XXIII, 910 pages) : 290 illustrations, 241 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides detailed technical protocols on current biomedical technologies and examples of their applications and capabilities. Chapters focus on molecular and cellular analytical methods, experimental new drug delivery approaches, guided surgery, implants and tissue engineering. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Biomedical Engineering Technologies, Volume 2 provides technical details in descriptions of major technologies by experts in the field.

Contents:

Development of a Multi-Target Protein Biomarker Assay for Circulating Tumor Cells

Method to Isolate Dormant Cancer Cells from Heterogeneous Populations

Label-Free Morphological Phenotyping of in vitro 3D Micro Tumors

High-throughput microenvironment microarray (MEMA) (file #high-resolution imaging

Real-Time Analysis of AKT Signaling Activities at Single-Cell Resolution Using Cyclic Peptide-Based Probes

Microfluidic device technologies for digestion, disaggregation, and filtration of tissue samples for single cell applications

Microdissection methods utilizing single cell subtype analysis and the impact on precision medicine

Functionalized lineage tracing for the study and manipulation of heterogeneous cell populations

Fluorescence lifetime imaging probes for cell-based measurements of enzyme activity

Assessment of intracellular GTP levels using genetically encoded fluorescent sensors

Node-Pore Sensing for characterizing cells and extracellular vesicles

Affinity-Based Enrichment of Extracellular Vesicles with Lipid Nanoprobes

Droplet magnetofluidic assay platform for quantitative methylation-specific PCR

Droplette: A Platform Technology to Directly Deliver Nucleic Acid Therapeutics and Other Molecules into Cells and Deep into Tissue Without Transfection Reagents

Molecular imaging of HER2 in patient tissues with touch prep-quantitative single molecule localization microscopy

Microchip free-flow electrophoresis for bioanalysis, sensing and purification

Green Chemistry Preservation and Extraction of Biospecimens for Multi-omic Analyses

TdT-UTP DSB End Labeling (TUDEL), for Specific, Direct in situ Labeling of DNA Double Strand Breaks

Ligand-directed GPCR Antibody Discovery

Self-Induced Back-Action Actuated Nanopore Electrophoresis (Sane) (File #Sensor For Label-Free Detection Of Cancer Immunotherapy-Relevant Antibody-Ligand Interactions

Incorporating, quantifying, and leveraging noncanonical amino acids in yeast

Nuclease-assisted, multiplexed minor-allele enrichment: application in liquid biopsy of cancer

Implementation of Ion Mobility Spectrometry-Based Separations in Structures for Lossless Ion Manipulations (SLIM)

Pleural Effusion Aspirate for use in 3D Lung Cancer Modeling and Chemotherapy Screening

Using Optical Tweezers to Dissect Allosteric Communication Networks in Protein Kinases

Focused ultrasound-mediated intranasal brain drug delivery technique (FUSIN)

Extracellular pH mapping as therapeutic readout of drug delivery in glioblastoma

Charge-Based Multi-Arm Avidin Nano-construct as a Platform Technology for Applications in Drug Delivery

Chemical Modification of Proteins and Their Intracellular Delivery Using Lipidoid Nanoparticles

Generation of Membrane-derived Nanovesicles by Nitrogen Cavitation for Drug Targeting Delivery and Immunization

Laboratory-scale production of sterile targeted microbubbles

Adeno-associated viral vector immobilization and local delivery from bare metal surfaces

Decellularization and Recellularization Methods for Avian Lungs: An Alternative Approach for Use in Pulmonary Therapeutics

Methods for Forming Human Lymphatic Microvessels In Vitro and Assessing Their Drainage Function

Polymer Based Micro-Nanostructured Scaffolds for Bone Tissue Engineering

Biodegradable electrospun nanofibrous scaffolds for bone tissue engineering

Bio-Tribometer for the Assessment of Cell and Tissue Toxicity of Orthopedic Metal Implant

Methods for Quantifying Neutrophil Extracellular Traps on Biomaterials

In Vivo Imaging of Implanted Hyaluronic Acid Hydrogel Biodegradation

Computational modeling and simulation to quantify the effects of obstructions on the performance of ventricular catheters used in hydrocephalus treatment

Selection of Cancer Stem Cell

targeting Agents Using Bacteriophage Display

Nano-Scintillator-Based X-Ray Induced Photodynamic Therapy

Methods to measure the inhibition of ABCG2 transporter and ferrochelatase activity to enhance aminolevulinic acid-protoporphyrin IX fluorescence-guided tumor detection and resection

Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug-Target Engagement

Tumor in vivo Imaging with a New Peptide-based Fluorescent Probe

Thermal Ablation Treatment for Cervical Precancer (Cervical Intraepithelial Neoplasia grade 2 or higher [CIN2+])

Employing Novel Porcine Models of Subcutaneous Pancreatic Cancer to Evaluate Oncological Therapies.

Other Format:

Printed edition:

ISBN:

978-1-0716-1811-0

9781071618110

Access Restriction:

Restricted for use by site license.