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Animal Coronaviruses / edited by Leyi Wang.

Format:
Book
Contributor:
Wang, Leyi, Editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Springer protocols (Series) 1949-2456
Springer Protocols Handbooks, 1949-2456
Language:
English
Subjects (All):
Virology.
Veterinary microbiology.
Veterinary Microbiology.
Local Subjects:
Virology.
Veterinary Microbiology.
Physical Description:
1 online resource (XIII, 327 pages) : 45 illustrations, 41 illustrations in color.
Edition:
2nd ed. 2022.
Contained In:
Springer Nature eBook
Place of Publication:
New York, NY : Springer US : Imprint: Humana, 2022.
System Details:
text file PDF
Summary:
This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluable Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. Thorough and hands-on, Animal Coronaviruses, Second Edition will help readers design and carry out their increasingly important projects in clinical research, diagnostics, disease surveillance, and epidemiological study involving this family of viruses.
Contents:
Animal Coronaviruses
Feline Coronavirus: Insights into the Pathogenesis and Diagnosis
Animal Models for Studying COVID-19 Pathology and Infection
Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samples
Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells
Isolation and Propagation of Porcine Deltacoronavirus in Cell Culture and Embryonated Chicken Eggs
Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs
Real-Time Reverse Transcription Polymerase Chain Reaction for Simultaneous Detection of Bovine Coronavirus and Other Pathogens
TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus
Real-Time RT-PCR for Detection of Feline Coronavirus
SARS-CoV-2 Real-Time RT-PCR Assay in Animals
Real-Time PCR and Antigen-Capture ELISA for the Diagnosis of Porcine Deltacoronavirus Infection
Identification of a Neutralizing Epitope-Containing Segment in Turkey Coronavirus Spike Protein for Diagnostic Serology
Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detecting Protective Antibodies to Turkey Coronavirus Based on Recombinant Spike Protein Segment with Neutralizing Epitope
ELISA, Virus Neutralization, and Immunofluorescence Assays for Detection of Antibodies Against Porcine Deltacoronavirus
Luminescent Immunoprecipitation System (LIPS) Development for the Specific Detection of PDCoV and SARS-CoV-2 Antibodies
Construction of a Mouse-Feline Chimeric Neutralizing Antibody Against SARS-CoV-2
Microneutralization Test of Avian Infectious Bronchitis Virus Using Vero Cells
Pig Bioassay to Assess Infectivity of Porcine Epidemic Diarrhea Virus Detected in Various Sample Matrices
Diagnostic Investigation and Confirmation of Emerging Turkey Coronaviral Enteritis
Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus
Whole-Genome Sequencing of Avian Infectious Bronchitis Virus by iSeq100 Platform and MinION
Phylogenetic and Recombination Analysis of Animal Coronaviruses.
Other Format:
Printed edition:
ISBN:
978-1-0716-2091-5
9781071620915
Access Restriction:
Restricted for use by site license.

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