Cell Polarity Signaling : Methods and Protocols / edited by Chenbei Chang, Jianbo Wang.

Format:

Book

Contributor:

Chang, Chenbei, Editor.

Wang, Jianbo, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2438

Methods in Molecular Biology, 1940-6029 ; 2438

Language:

English

Subjects (All):

Cytology.

Polarity (Biology).

Cell Polarity.

Cell Biology.

Local Subjects:

Cell Polarity.

Cell Biology.

Physical Description:

1 online resource (XIV, 530 pages) : 124 illustrations, 105 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume explores detailed methods to investigate various aspects of biology related to cell polarity, or asymmetry within a cell. Molecular, cellular, and tissue-level regulation and function as well as diseases caused by impairment of cell polarity are explored by these methods. Beginning with advanced imaging and biochemical methods, the book continues with planar cell polarity (PCP) signaling in morphogenesis in diverse developmental contexts, apicobasal (AB) cell polarity in development and diseases, as well as directional cell migration and biomechanics in cell polarity. The collection includes the usage of a wide variety of model systems and an extensive array of techniques, including genetic, imaging, biochemical, and biomechanical. Written for the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Cell Polarity Signaling: Methods and Protocols aims to enable researchers to delve into the stimulating field of cell polarity and contribute to our understanding of how coordinated control of protein stability, trafficking, membrane retention, post-translational modification, and dynamic organization leads to active regulation of cell polarity. Chapter 29 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Use of Fluorescence Recovery after Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins

FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs

Live‐Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics

Single-Cell Single Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates

Biochemical Assays to Detect Activation of Small GTPases Rho, Rac, and Cdc42 during Morphogenesis

Analysis of Planar Cell Polarity Complexes by Proximity Biotinylation in Xenopus Embryos

Regulation of Cell Polarity by Posttranslational Protein Palmitoylation

Enrichment and Detection of Wnt Proteins from Cell Culture Media

Using Live-Imaging to Examine Early Cardiac Development in Zebrafish

Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm

In Ovo Gain- and Loss-of-Function Approaches to Study Gut Morphogenesis

Avian Embryos as a Model to Study Vascular Development

BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice

Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics

Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embryo

Methods to Investigate Cell Polarity of Inner Ear

Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice

In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance

Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance

Dissection, Fixation, and Immunostaining of the Drosophila Midgut

In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands

Imaging Epidermal Cell Rearrangement in the C. elegans Embryo

Methods for the Study of Apical Constriction during Ascidian Gastrulation

Assays for Apical Constriction Using the Xenopus Model

The Use of 3-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation

Studying Cell Polarity Dynamics during Cancer Initiation Using Inducible 3D Organotypic Cultures

Under-Agarose Chemotaxis and Migration Assays for Dictyostelium

Mapping Asymmetry in Collective Cell Migration: Lessons from Border Cells in Drosophila Oogenesis

Targeted Injections, Atomic Force Microscopy, and Hydrogels: A Toolbox to Study Tissue Mechanics In Vivo and Ex Vivo

Quantitative Analysis of Directional Neural Crest Cell Migration.

Other Format:

Printed edition:

ISBN:

978-1-0716-2035-9

9781071620359

Access Restriction:

Restricted for use by site license.